Présentation Groupe
Responsable de groupe : Franck Fieschi
The group is mainly focused on the molecular investigation of membrane proteins related to pathogen fitness, pathogen-host interactions and immune response. For this, we notably develop new strategies and expertise in membrane protein biochemistry and biophysics. Essential soluble proteins found exclusively in pathogens are also studied. The group is composed of three teams with complementary skills, expertise in membrane protein biochemistry, and biophysics.
- Conserved ATP/GTPase Team (J.M. Jault) : CAGT.
- Membrane and Immunity Team (F. Fieschi) : MIT.
- Structure and Stability of Integral Membrane Protein in Aqueous Solution (C. Ebel) : SSIMPA.
Thèmes de recherche :
- Bacterial ATP-Binding Cassette multidrug transporters.
- New essential bacterial enzymes.
- Neutrophilic NADPH oxidase complex.
- C-type lectin receptors of dendritic cells.
- Development of GPCR expression and production strategies in E.coli.
- New surfactants for the in vitro study of membrane proteins.
- Structural study of membrane proteins related to virulence and pathogenicity.
Techniques utilisées :
- Biochemistry of membrane and soluble proteins.
- Cell free protein expression.
- Automation in protein purification.
- Surface plasmon resonance.
- Fluorescence.
- Analytical ultracentrifugation.
- Size exclusion chromatography/light scattering.
- Small angle X-Rays and neutrons scattering.
Services disponibles :
Publications 2011
Andreini M, Doknic D, Sutkeviciute I, Reina JJ, Duan J, Chabrol E, Thepaut M, Moroni E, Doro F, Belvisi L, Weiser J, Rojo J, Fieschi F, Bernardi A
Second generation of fucose-based DC-SIGN ligands : affinity improvement and specificity versus Langerin.
Org Biomol Chem (2011) 9 : 5778-86
Brown PH, Balbo A, Zhao H, Ebel C, Schuck P
Density contrast sedimentation velocity for the determination of protein partial-specific volumes.
PloS one (2011) 6 : e26221.
Cadavid-Restrepo G, Gastardello TS, Faudry E, Silva H, Bastos IMD, Negreiros RS, Lima MM, Assumpção TC, Almeida KC, Ragno M, Ebel C, Ribeiro BM, Felix CR, Santana JM
The major leucyl aminopeptidase of Trypanosoma cruzi (LAPTc) assembles into a homohexamer and belongs to the M17 family of metallopeptidases.
BMC Biochemistry (2011) 12 : 46
Dezi M, Fribourg P-F, Di Cicco A, Jault J-M, Chami M, Lévy D
Binding, reconstitution and 2D crystallization of membrane or soluble proteins onto functionalised lipid layer observed in situ by reflected light microscopy.
J. Struct. Biol (2011) 174 : 307-314
Ebel C
Sedimentation velocity to characterize surfactants and solubilized membrane proteins.
Methods (2011) 54 : 56–66
El Ghachi M, Mattei PJ, Ecobichon C, Martins A, Hoos S, Schmitt C, Colland F, Ebel C, Prevost MC, Gabel F, England P, Dessen A, Boneca IG
Characterization of the elongasome core PBP2 : MreC complex of Helicobacter pylori.
Mol.microbiol. (2011) 82 : 68-86
Galián C, Manon F, Dezi M, Torres C, Ebel C, Lévy D, Jault J-M
Optimized purification of a heterodimeric ABC transporter in a highly stable form amenable to 2-D crystallization.
PLoS One (2011) 6 : e19677
Guzzi C, Angulo J, Doro F, Reina JJ, Thépaut M, Fieschi F, Bernardi A, Rojo J, Nieto PM
Insights into molecular recognition of Lewis(X) mimics by DC-SIGN using NMR and molecular modelling.
Org Biomol Chem (2011) 9 : 7705-12
Jaquet V, Marcoux J, Forest E, Leidal KG, McCormick S, Westermaier Y, Perozzo R, Plastre O, Fioraso-Cartier L, Diebold B, Scapozza L, Nauseef WM, Fieschi F, Krause KH, Bedard K
NADPH oxidase (NOX) isoforms are inhibited by celastrol with a dual mode of action.
Br J Pharmacol (2011) 164 : 507-20
Luczkowiak J, Sattin S, Sutkevičiūtė I, Reina JJ, Sánchez-Navarro M, Thépaut M, Martínez-Prats L, Daghetti A, Fieschi F, Delgado R, Bernardi A, Rojo J
Pseudosaccharide functionalized dendrimers as potent inhibitors of DC-SIGN dependent Ebola pseudotyped viral infection.
Bioconjug Chem (2011) 22 : 1354-65
Matar-Merheb R, Rhimi M, Leydier A, Huché F, Galián C, Desuzinges-Mandon E, Ficheux D, Flot D, Aghajari N, Kahn R, Di Pietro A, Jault J-M, Coleman A, Falson P
Structuring detergents for extracting functional membrane proteins.
PLoS One (2011) 6 : e18036
Moriscot C, Gribaldo S, Jault J-M, Krupovick M, Arnaud J, Jamin M, Schoehn G, Forterre P, Weissenhorn W, Renesto P
Crenarchaeal CdvA forms double-helical filaments containing DNA and interacts with ESCRT-III-like CdvB.
PLoS One (2011) 6 : e21921
Park K-H, Billon-Denis E, Dahmane T, Lebaupain F, Pucci B, Breyton C, Zito F
In the cauldron of cell-free synthesis of membrane proteins : playing with new surfactants.
New Biotechnology (2011) 28 : 255-261
Popot J-L, Althoff T, Bagnard D, Baneres J-L, Bazzacco P, Billon-Denis E, Catoire LJ, Champeil P, Charvolin D, Cocco MJ, Cremel G, Dahmane T, de la Maza LM, Ebel C, Gabel F, Giusti F, Gohon Y, Goormaghtigh E, Guittet E, Kleinschmidt JH, Kuhlbrandt W, Le Bon C, Martinez KL, Picard M, Pucci B, Sachs JN, Tribet C, van Heijenoort C, Wien F, Zito F, Zoonens M
Amphipols from a to z.
Annual review of biophysics (2011) 40 : 379-408
Sabatte J, Faigle W, Ceballos A, Morelle W, Rodrígues CR, Lenicov FR, Thépaut M, Fieschi F, Malchiodi E, Fernández M, Arenzana-Seisdedos F, Lortat-Jacob H, Michalski JC, Geffner J, Amigorena S
Semen clusterin is a novel DC-SIGN ligand.
J Immunol (2011) 187 : 5299-309
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