LPC presentation

The LPC is studying the mechanisms involved in the equal distribution of chromosomes during mitosis and how these mechanisms are regulated by centromere/kinetochore associated proteins. Our work is coupled with studies on small molecule inhibitors which exert their anti-mitotic function by targetting key processes involved in cell division.

Research topics

• Activation of spindle assembly checkpoint,
• Chromosome passenger proteins,
• Mechanisms of action of small molecule inhibitors with anti-mitotic activities.

Techniques

• siRNA mediated suppression of protein expression,
• Confocal microscopy,
• Flow cytometry,
• Molecular biology,
• Protein purification,
• Biochemical analysis,
• FPLC.

Major Publications

Scrittori, L*, Skoufias, DA*, Hans F, Gerson V, Sassone-Corsi, P , Dimitrov S, and Margolis RL. (2005) A Small C-terminal Sequence of Aurora B is Responsible for Localization and function. Molecular Biology of the Cell 16:292-305. (Rated as excellent in Faculty 2000).

Skoufias DA, Lacroix FB, Andreassen, PR, Wilson L, Margolis RL. (2004) Inhibition of DNA decatenation, but not DNA damage, arrests cells at metaphase. Molecular Cell 15:1-20.

Skoufias DA, Andreassen PR, Lacroix FB, Wilson L, Margolis RL. (2001) Mammalian MAD2 and Bub1/BubR1 respectively recognize spindle attachment and kinetochore tension. PNAS 98:4492-4497.

Skoufias DA, Mollinari C, Lacroix FB, Margolis RL. (2000) Human survivin is a kinetochore-associated passenger Protein. Journal of Cell Biology 151:1575-1582.

Chantalat L*, Skoufias DA*, Kleman JP, Jung B, Dideberg O, and Margolis RL. (2000) Crystal structure of human survivin reveals a bow-tie shaped dimer with two unusual alpha helical extensions. Molecular Cell 6:183-189.

(The list of all the publications of the laboratory is available here)