Presentation of the LPM
Director : Eva PEBAY-PEYROULA; co-supervisor : Andrea Dessen
Introduction
Through a large variety of biological systems studied in the lab, we develop an expertise in the study of proteins in general, and more specifically of membrane proteins. Our skills involve the expression, purification, biochemical/biophysical characterization of proteins (enzymatic activities, transport…) as well as methodological developments in crystal growth and protein crystallography. Our goals are to understand, at the molecular level, the mechanisms of proteins from different functional categories (enzymes, receptors, transporters, channels or nano-machines) and that originate from various living species, from bacteria to humans.
Research themes
- Methodological and technical developments for membrane protein structure determination,
- Immunity (NADPH oxydase, BLT1),
- Host-pathogen interactions (viral receptors, HIV and dengue viruses…),
- The Type III secretion system of major human pathogens (Pseudomonas aeruginosa and Escherichia coli),
- Virulence factors and cell division proteins from Streptococcus pneumoniae,
- New bacterial targets for future antibio-therapies
- Mitochondrial and ABC (‘ATP-Binding Cassette’) transporters,
- Molecular mechanisms and engineering of ion channels
- Heavy metals resistance mechanisms,
- genome preservation,
- Protein maturation in Gram-negative bacteria.
Key words
Membrane proteins – supramolecular complexes – protein-protein interactions - crystallization - lipid cubic phase - 3D structure - structure-function relationships - synchrotron radiation
Technical skills
- Molecular biology; overexpression and purification of recombinant proteins,
- Functional characterization of proteins by biophysical approaches (Surface Plasmon Resonance, Fluorescence, small angle scattering, analytical ultracentrifugation, circular dichroism…),
- Electrophysiology (patch-clamp, two-microelectrode voltage clamp),
- Crystallization of soluble and membrane proteins, as well as of supramolecular complexes
- X-ray crystallography.
Publications of the LPM
(The list of all the publications of the laboratory is available here)
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