Protein Analysis On Line

Size-exclusion chromatography coupled to light scattering, refractive index and/or absorbance detection combines the separation of macromolecules in solution and the analysis of their transportation in view of a rigorous thermodynamics. It allows:

• quantification of sample homogeneity
• determining association state of complexes including complex multi-component systems, i.e. protein plus detergent, RNA or polymer. It is particularly suitable to analyse membrane or glycosylated proteins.

Key words

High-pressure liquid chromatography, size-exclusion chromatography, static light scattering, homogeneity, molar mass, composition.

Contact

Christine EBEL

Specific equipment

High-pressure liquid chromatography: LC20 AD (Shimazu) Diode array UV detector: SPDM-20A (Shimazu) Refractometer: Optilab Rex (Wyatt) Multiangle static light scattering detector: miniDAWN TREOS (Wyatt)

Location

IBS

Additional informa

tion PAOL will be operational as a platform in 2010.