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Institut de Biologie StructuraleGrenoble / France

Highlights

How to control a reactive radical species

Combining X-ray crystallography, EPR spectroscopy and theoretical calculations, researchers from IBS-METALLO and INAC-CAMPS have determined the fine mechanism for carboxyl radical migration during methylindolic acid biosynthesis. A preorganized active site and restrained motions are essential to afford an efficient reaction.

Radical S-Adenosyl-l-methionine Tryptophan Lyase (NosL): How the Protein Controls the Carboxyl Radical •CO2- Migration. Amara P, Mouesca JM, Bella M, Martin L, Saragaglia C, Gambarelli S, Nicolet Y. J Am Chem Soc. 2018 Nov 16. doi: 10.1021/jacs.8b09142

The main target of HIV studied from every angle

By closely studying CCR5, one of the entry points of HIV into cells, researchers from Inserm, the Institut Pasteur, the University of Toulouse III - Paul Sabatier, the CNRS and IBS, demonstrate that its morphology determines the propensity of the virus to infect the body. This work supported by the ANRS and published in the Plos Pathogens journal is a new step towards understanding the role of CCR5 in HIV infection and as a target for blocking the entry of the virus into cells (details).

CCR5 structural plasticity shapes HIV-1 phenotypic properties. Colin P, Zhou Z, Staropoli I, Garcia-Perez J, Gasser R, Armani-Tourret M, Benureau Y, Gonzalez N, Jin J, Connell BJ, Raymond S, Delobel P, Izopet J, Lortat-Jacob H, Alcami J, Arenzana-Seisdedos F, Brelot A, Lagane B. PLoS Pathog. 2018 Dec 6;14(12):e1007432. doi: 10.1371/journal.ppat.1007432. eCollection 2018 Dec.

Innate immune protein C1q aggregates nanodiamonds and modifies macrophage response

Nanodiamonds (NDs) have many potential biomedical applications, such as cancer therapy, deep brain stimulation, and dentistry. In collaboration with the Service de Chimie Bioorganique et de Marquage (SCBM, CEA, Université Paris-Saclay), IBS researchers have shown that, even though C1q does not trigger the immune complement system cascade, its interaction modifies phagocytosis and cytokine response to NDs and may interfere with the multiple physiological and pathological processes that involve C1q.

Recognition protein C1q of innate immunity agglutinates nanodiamonds without activating complement. Belime A, Thielens NM, Gravel E, Frachet P, Ancelet S, Tacnet P, Caneiro C, Chuprin J, Gaboriaud C, Schoehn G, Doris E, Ling WL. Nanomedicine-Nanotechnology, Biology and Medicine; doi: 10.1016/j.nano.2018.09.009