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Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / LE ROY Aline / THEPAUT Michel

Differential Scanning Fluorimeter

Equipment

Prometheus NT.48 (Nanotemper).

Summary

This instrument allows to evaluate protein thermal and chemical stability by determining the unfolding transition midpoint (Tm) and the aggregation of the protein. This is done following changes in intrinsic Trp fluorescence (by differential scanning fluorimetry: DSF) and by detecting scattering of light.

It allows to screen up to 48 conditions in parallel, using 12µL of sample per assay with a concentration between 5 µg/mL to 250 mg/mL (optimum is around the mg/mL). A classical set of measure lasts around 1h30.

Keywords

Thermal Shift Assay, melting point, Trp fluorescence, aggregation, high resolution.

Contacts

- Aline Le Roy
- Michel Thépaut

Access mode

The access to Prometheus is open to PSB members, but not free.

Before his first use, each new user has to be trained in the use of the system by Michel Thépaut or Aline Le Roy. After signature of the charter of use, he will be able to book and use the instrument. All experiments and analyses have to be performed by users.

All uses of the system have to be noted on the dedicated agenda, which has to be used also to book the system.

Cost

Capillaries have to be brought by users (available at IBS store or contact Pierre Soule (pierre dot soule at nanotemper dot de).
Standard Capillaries (x400) ref: PR-C002
High Sensitivity (x200) ref: PR-C006

No additional cost.

Location

IBS, 2nd floor, room 327.