Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / FAVIER Adrien / SCHANDA Paul

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Research internship

Research internship (BTS) Protein Purification
We are offering an internship on protein purification ("niveau Bac+2"). See attached document for information :

Post-doc and/or PhD positions available :

Solid-state NMR studies of interactions, dynamics and function in molecular chaperones

The biomolecular NMR team at the Structural Biology Institute in Grenoble, France, (team leader Paul Schanda) has openings for post-doctoral/PhD researchers to work on elucidating interactions and dynamics in chaperone complexes, in research project funded by the European Research Council (ERC project ProtDyn2Function).

We are seeking candidates for a project that aims at characterizing molecular interactions and dynamics in complexes of large chaperones with unfolded/misfolded protein targets. In this project we use the solid- as well as solution-state NMR to probe molecular interactions between a hundreds-of-kilodalton large chaperone complex and “client” proteins, as well as structural and dynamical features of these complexes. The protein targets are already well established in our hands, and existing NMR characterizations/assignments as well as high-resolution EM data allow rapidly focusing on the biological/biophysical questions.

We are seeking highly motivated and creative candidates with a strong ability to work effectively and congenially with other group members.
For post-doctoral candidates, experience in biomolecular NMR spectroscopy, as well as protein production and purification are required. Strong background in chaperones would be an advantage, but not required. Experience in one of the following research areas are a strong plus : programming and data analysis (e.g. python programming), biochemical characterization of proteins and functional assays, spectroscopic techniques.
Applications for a PhD position are also welcome ; experience in NMR spectroscopy is not mandatory for the PhD position, and the successful candidate should be highly motivated to learn state-of-the-art biomolecular solid-state NMR. Experience in one of the following research areas are a strong plus : programming and data analysis (e.g. python programming), biochemical characterization of proteins and functional assays, spectroscopic techniques.

The successful candidate will be part of a dynamic recently established research team, hosted in the Structural Biology Institute (IBS), in one of Europe’s most vibrant structural biology communities (centered around the European Synchrotron Facility, the ILL neutron source, EMBL Grenoble). Our group has access to brand-new NMR equipment for both solution- and solid-state NMR (2x600 MHz, 700, 850, 950 MHz with cryoprobes and fast-spinning MAS probes), a state-of-the-art electron microscopy facility, as well as a large panel of complementary biophysical techniques. The candidate will benefit from extensive expertise on advanced isotope labeling, solution- and solid-state NMR and biochemistry in the NMR group. Grenoble is a pleasant mid-sized city located at the heart of the French alps, with (public) transport connections to Paris (3 h), the mediterranean and the nearby mountain resorts.

Applications, containing a detailed CV, a list of publications and a clear statement of motivation, as well as names of 2-3 reference persons should be sent via e-mail to Paul Schanda

Selected recent publications :
Observing the overall rocking motion of a protein in a crystal.
Ma P, Xue Y, Coquelle N, Haller JD, Yuwen T, Ayala I, Mikhailovskii O, Willbold D, Colletier JP, Skrynnikov NR, Schanda P.
Nature Communications. 2015 ; 6:8361

Probing transient conformational states of proteins by solid-state R(1ρ) relaxation-dispersion NMR spectroscopy.
Ma P, Haller JD, Zajakala J, Macek P, Sivertsen AC, Willbold D, Boisbouvier J, Schanda P.
Angewandte Chemie Int Ed Engl. 2014 ; 53(17):4312-7.

Atomic model of a cell-wall cross-linking enzyme in complex with an intact bacterial peptidoglycan.
Schanda P, Triboulet S, Laguri C, Bougault CM, Ayala I, Callon M, Arthur M, Simorre JP.
Journal of the American Chemical Society. 2014, 136(51):17852-60.

Master’s students and PhD students.

We are constantly looking for motivated students that are interested in the development and application of NMR spectroscopy in structural biology for an internship (Master). We are working on a number of different research topics (see dedicated sections on group’s main page). Depending on the candidate’s background and interest, as well as current possibilities in the lab, we propose internships or PhD opportunities in various fields, such as :

* structure determination using solution-state NMR, particularly in the context of proteins involved in the bacterial cell wall

* dynamics studies, both by solution- and solid-state NMR, including the development of novel experimental techniques.

* kinetic NMR and protein folding studies.

Please feel free to inquire about the current possibilities as well as about details of our research.

Experience in expression and puri­fi­ca­tion of proteins, NMR spectroscopy in solution or solids, data analysis and programming are welcome. The applicant should ideally have a degree in biochemistry, biophysics, chemistry or a related discipline, and have a strong motivation in research and creative thinking.

We encourage applicants from outside Grenoble, or outside France, and may also host students wishing to perform a research internship in the context of an ERASMUS exchange.

Please contact the team leaders for informal queries and applications :

Bernhard Brutscher
Jerome Boisbouvier
Paul Schanda
Jean-Pierre Simorre

Clearly state your motivation to work on this particular research field, describe a detailed curriculum and possibly provide references.