Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / Pebay-Peyroula Eva

Membrane Transporters Group

Group leader: Eva Pebay-Peyroula

The group aims to understand membrane protein activity mechanisms at molecular and atomic level. The group is interested in high resolution structure determination, functional characterization and new approaches for membrane protein crystallization.

The membrane transporter group is composed of 2 teams :

Key words

Production, characterization and crystallization of membrane proteins, Nucleotide transporter, AAC, NTT, Cys-loop transporter family, Serotonin receptor 5-HT3, Microorganism rhodopsins, Connexines

Specialized techniques

  • Overexpression (prokaryotes and eukaryotes systems) and purification of membrane proteins
  • Biophysical, biochemical and functional characterizations (thermal stability, FTIR, transport)
  • Membrane protein crystallization with vapor diffusion and lipidic phase (platform)
  • X-ray crystallography

Major publications

- Gushchin I, Shevchenko V, Polovinkin V, Kovalev K, Alekseev A, Round E, Borshchevskiy V, Balandin T, Popov A, Gensch T, Fahlke C, Bamann C, Willbold D, Bueldt G, Bamberg E, Gordeliy V (2015) Nature Structural and Molecular Biology 22 (5).

- Nogly P, Gushchin I, Remeeva A, Esteves A, Borges N, Ma P, Ishchenko A, Grudinin S, Round E, Moraes I, Borshchevskiy V, Santos H, Gordeliy V, and Archer M (2014), Nature Communications 5:4169.

- Hassaine G, Deluz C, Grasso L, Wyss R, Tol MB, Hovius R, Graff A, Stahlberg H, Tomizaki T, Desmyter A, Moreau C, Li XD, Poitevin F, Vogel H, Nury H. (2014) Nature 512(7514), 276-8.

- Moukhametzianov R, Klare JP, Efremov R, Baeken C, Göppner A, Labahn J, Engelhard M, Büldt G, Gordeliy VI. (2006) Nature 440(7080), 115-119.

- Gordeliy VI, Labahn J, Moukhametzianov R, Efremov R, Granzin J, Schlesinger R, Büldt G, Savopol T, Scheidig AJ, Klare JP, Engelhard M. (2002) Nature 419(6906), 484-487.

- E.Pebay-Peyroula, C. Dahout-Gonzalez, R. Kahn, V. Trézéguet, G.J.-M. Lauquin and G. Brandolin (2003) Nature 426, 39-44.

(You can find all the publications of the group here).