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Institut de Biologie StructuraleGrenoble / France

Contacts relatifs à cet article / PELLEQUER Jean-Luc

AFM team presentation

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Members

Team leader : Jean-Luc Pellequer (CEA Researcher, HDR)

Team members :

- Jean-Marie Teulon (CEA Engineer)
- Jean-Luc Pellequer (CEA Researcher) ORCID iD iconorcid.org/0000-0002-8944-2715
- Daphna Fenel (CEA Technician, 20%)
- Shu-wen W. Chen (CDD Researcher)
- Harinderbir Kaur (Master 2 student)

Presentation

The AFM team aims at developing analyses techniques in atomic force microscopy (AFM) in three major axes : high-resolution imaging, dynamic force spectroscopy and cellular biomechanics. Current projects are :

  • Bionanomechanics in plant —> A European Training Network grant has been awarded to a consortium of researchers (Phys2BioMed) mainly originating from our previous COST Action TD1002. The project aims at studying the mechanical response of root tissues during abiotic stress for understanding the STOP1-LPR1 multi-stress signaling pathways in Arabidopsis plants.
  • Methodological developments —> The long-term activity in method developments such as image processing will be pursued. New developments for analyzing force-indentation curves are developed. This is important to extract the Young modulus (elasticity) of cells for which the current lack of accurate automated programs is a handicap to develop novel measurement of complicated systems such as live plant roots.
  • Metrology and nanotoxicology —> The team is participating to a national inter-laboratory comparison of techniques to determine the size of nanoparticles. The team is a co-leader on the AFM technique.

For more information, please contact Jean-Luc Pellequer

Keywords

- Atomic Force Microscopy, Dynamic Force Spectroscopy, Nanomechanics, Image processing, Nanoparticle metrology, AFM-based structure reconstruction, Comparative modeling.

Team Instruments

- Multimode 8, Nanoscope V (Bruker)
- Dimension 3100, Nanoscope V (Bruker)
Instruments associated to microscopes :
- Microscope fluo, Nikon Eclipse TE2000
- UV/Ozone lamp
- Peltier-cooled incubator Memmert IPP110+ with a full spectrum LED light 45W

Major publications

  • Silvestre-Roig C, Braster Q, Wichapong K, Lee EY, Teulon JM, Berrebeh N, Winter J, Adrover JM, Santos GS, Froese A, Lemnitzer P, Ortega-Gómez A, Chevre R, Marschner J, Schumski A, Winter C, Perez-Olivares L, Pan C, Paulin N, Schoufour T, Hartwig H, González-Ramos S, Kamp F, Megens RTA, Mowen KA, Gunzer M, Maegdefessel L, Hackeng T, Lutgens E, Daemen M, von Blume J, Anders H-J, Nikolaev VO, Pellequer JL, Weber C, Hidalgo A, Nicolaes GAF, Wong GCL and Soehnlein O (2019) Externalized histone H4 orchestrates chronic inflammation by inducing lytic cell death. Nature 569 : 236-240.
  • Balzergue C, Dartevelle T, Godon C, Laugier E, Meisrimler C, Teulon JM, Creff A, Bissler M, Brouchoud C, Hagège A, Müller J, Chiarenza S, Javot H, Becuwe-Linka N, David P, Péret B, Delannoy E, Thibaud M-C, Armangaud J, Abel S, Pellequer JL, Nussaume L and Desnos T (2017) Low phosphate activates STOP1-ALMT1 to rapidly inhibit root cell elongation. Nat. Commun. 8 : 15300.