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Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / NEUMANN Emmanuelle
Contact person(s) related to this article / Benoit Gallet

Microscope Tecnai POLARA (FEI)

Polara FEG Microscope - 300 kV

Specificities :
- Tension : 100 - 300 kV
- Field Emission Gun (FEG)
- K2 Summit GATAN electron counting direct detection camera
- 2kx2k GATAN GIF
- Software : Tomography FEI (FEI), Xplore3D (FEI), GMS 3.2 (GATAN - automatic data collection)

Since February 2010, the IBS has a Polara transmission electron microscope for high-resolution cryo-electron microscopy. The microscope was funded by generous grants from IBISA, CNRS, EMBL and the Région
Rhône-Alpes and its acquisition has only been possible because IBS (UMR 5075), UVHCI (UMI 3265) and EMBL Grenoble Outstation have put effort together to get it. Electron microscopists from the Structural Biology Partnership have access to this microscope.
This microscope is a cryo-transmission EM with a 300 kV field emission gun (FEG), which allows imaging of proteins in their native state. The microscope stage is stable to allow high-resolution imaging. The design «cryo» also gives a low rate of specimen contamination in the microscope (one can work on the same grid for several days).
The Polara can be loaded with 6 samples, for convenient screening of multiple samples. The microscope has reliable grid loading and specimen transfer (achieved through a protected environment in the loading bay, and the transfer system under vacuum).

To collect high resolution data, the microscope is now fitted with one of the highest performance detector available for cryo-electron microscopy: the K2 Summit electron counting direct detection camera (GATAN). The Tecnai G2 Polara is also fitted with a 2k x 2k Gatan post column energy filter.

The Polara is also equipped to do single tilt electron tomography (collecting a series of tilted images +/-70 deg) with:

- The following tomography imaging options:
Gatan K2 Summit,
Gatan Imaging Filter 2002 (2k X 2k Tridiem GIF)

- The following software:
FEI Tomography (FEI),
Xplore3D (FEI)
GMS 3.2 (automatic data collection - GATAN)

The Polara microscope is open to outside (national and international) users after approval of their scientific projects. Trained cryo-electron microscopists have priority but other projects can be accommodated within the framework of collaboration. In any case if you want to use or have an access to the Polara Microscope or to another equipment of the electron microscopy platform, please send an email to : ibs-plateforme-em.contact@ibs.fr.