Institut de Biologie StructuraleGrenoble / France

Contacts relatifs à cet article / NEUMANN Emmanuelle
Contacts relatifs à cet article / Benoit Gallet

Microscope GLACIOS (Thermo Scientific)

Specificities :
- Tension : 200 kV
- Field Emission Gun (FEG)
- Falcon II Thermo Scientific electron counting direct detection camera
- CETA CMOS camer
- Software : Tomography TEM, EPU (automatic data collection)

December 2018, the IBS received a GLACIOS transmission electron microscope for high-resolution cryo-electron microscopy. The microscope was funded by generous grants from CEA, CNRS, ESRF and FRISBI.
This microscope is a cryo-transmission EM with a 200 kV field emission gun (FEG), which allows imaging of proteins in their native state.
The microscope is equipped with an "AutoLoader" system allowing to load grids. The Autoloader system allows to load up to 12 grids inside the column of the microscope in an automatic way.

To collect high resolution data, the microscope is fitted with one of the highest performance detector available for cryo-electron microscopy : the Falcon II electron counting direct detection camera (Thermo Scientific).

The GLACIOS is also equipped to do single tilt electron tomography with :
- Falcon II camera (direct detection camera)
- Tomography TEM software

The GLACIOS microscope is open to outside (national and international) users after approval of their scientific projects. Trained cryo-electron microscopists have priority but other projects can be accommodated within the framework of collaboration. In any case if you want to use or have an access to the Polara Microscope or to another equipment of the electron microscopy platform, please send an email to :