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Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / ROYANT Antoine

The icOS team (A. Royant)

In crystallo Optical Spectroscopy (icOS) Team

icOS members

  • Permanent:
    Antoine Royant (CR1 CNRS)
    Martin Byrdin (CEA staff scientist)
    Philippe Jacquet (AI CNRS)
  • Non-permanent:
    Sylvain Aumonier (PhD student)
    Damien Clavel (PhD student)
    Guillaume Gotthard (postdoctoral fellow)

Presentation

The icOS Team has two main ijtereqtc. On one hand, we manage the development and operation of the in crystallo optical spectroscopy platform Cryobench located at the ESRF (see Cryobench website), which allows user to assess the functional state of their protein in the crystalline state, monitor the extend of X-ray radiation damage and perform kinetic crystallography experiments. On the other hand we study various types of fluorescent proteins using a combination of structural biology and biophysical techniques with the idea of finding ways to improve their fluorescence properties. This fluorescent proteins are of the GFP type (mTurquoise2 ([bleu ciel]cyan[/bleu ciel] colour), mNeonGreen ([vert clair]yellow-green[/vert clair]), mScarlet ([rouge]red[/rouge]), phytochrome type (IFP2.0, iBlueberry ([marron]infrared[/marron]) or flavin-binding type (miniSOG (dual functionalities: [vert]green[/vert] fluorescence and generation of reactive oxygen species)).

Cryobench laboratory is available on demand (click A three-dimensional movie of structural changes in bacteriorhodopsin. Nango E, Royant A, Kubo M, Nakane T, Wickstrand C, Kimura T, Tanaka T, Tono K, Song C, Tanaka R, Arima T, Yamashita A, Kobayashi J, Hosaka T, Mizohata E, Nogly P, Sugahara M, Nam D, Nomura T, Shimamura T, Im D, Fujiwara T, Yamanaka Y, Jeon B, Nishizawa T, Oda K, Fukuda M, Andersson R, Båth P, Dods R, Davidsson J, Matsuoka S, Kawatake S, Murata M, Nureki O, Owada S, Kameshima T, Hatsui T, Joti Y, Schertler G, Yabashi M, Bondar AN, Standfuss J, Neutze R, Iwata S (2016) Science ; 354(6319):1552-1557
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Structural analysis of the bright monomeric yellow-green fluorescent protein mNeonGreen obtained by directed evolution. Clavel D, Gotthard G, von Stetten D, De Sanctis D, Pasquier H, Lambert G G, Shaner N C, and Royant (2016) Acta Cryst. D 72, 1298-1307

mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Bindels D S, Haarbosch L, van Weeren L, Postma M, Wiese K E, Mastop M, Aumonier S, Gotthard G, Royant A, Hink M & Gadella T W Jr (2016) Nat. Methods 14 (53-56)
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Structural Determinants of Improved Fluorescence in a Family of Bacteriophytochrome-Based Infrared Fluorescent Proteins: Insights from Continuum Electrostatic Calculations and Molecular Dynamics Simulations. Feliks M, Lafaye C, Shu X, Royant A, Field M. (2016) Biochemistry 55 (31)

In crystallo optical spectroscopy (icOS) as a complementary tool on the macromolecular crystallography beamlines of the ESRF. von Stetten D, Giraud T, Carpentier P, Sever F, Terrien M, Dobias F, Juers D H, Flot D, Mueller-Dieckmann C, Leonard G A, de Sanctis D & Royant A (2015). Acta Cryst. D 71, 15-26

Direct evidence for a peroxide intermediate and a reactive enzyme-substrate-dioxygen configuration in a cofactor-free oxidase. Bui S, von Stetten D, Jambrina PG, Prangé T, Colloc’h N, de Sanctis D, Royant A, Rosta E, Steiner RA. (2014) Angew Chem Int Ed Engl. 53 (50).

An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Yu D, Gustafson WC, Han C, Lafaye C, Noirclerc-Savoye M, Ge W P, Thayer D A, Huang H, Kornberg T B, Royant A, Jan LY, Jan Y N, Weiss W A, Shu X. (2014) Nat Commun. 5:3626

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink M A, van Weeren L, Gadella T W Jr, Royant A. (2012) Nat Commun. 3:751
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