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Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / ROYANT Antoine

The icOS team (A. Royant)

update 2019/02/18

Members

  • Permanent :
    Antoine Royant (DR2 CNRS)
    Martin Byrdin (CEA staff scientist)
    Philippe Jacquet (AI CNRS)
    Jérôme Dupuy (assistant professor UGA)
  • Non-permanent :
    Janek Bzdrenga (postdoctoral fellow)
    Sylvain Aumonier (PhD student)
    Kevin Duarte (Master 2 student)
  • Alumni :
    Guillaume Gotthard 2014 - 2019
    Damien Clavel 2013 - 2017
    Céline Lafaye 2012-2015
    Maxime Terrien 2011 - 2014
    Gaëlle Batot 2010-2013
    David von Stetten 2010-2013

Presentation

We study various types of fluorescent proteins using a combination of structural biology and biophysical techniques with the idea of finding ways to improve their fluorescence properties. This fluorescent proteins are of the GFP type (mTurquoise2, mNeonGreen, mScarlet), phytochrome type (IFP2.0, iBlueberry) or flavin-binding type (miniSOG (dual functionalities : fluorescence and generation of reactive oxygen species)).

The team also supports the operation of the in crystallo Optical Spectroscopy Lab, which is located at the ESRF. See : http://www.esrf.eu/home/UsersAndScience/Experiments/MX/icOS.html

Keywords

In crystallo optical spectroscopy, X-ray crystallography, kinetic crystallography, time-resolved crystallography, fluorescent protein, protein dynamics and mechanisms, methods developments.

Key publications

Tailing miniSOG : structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein. Torra J, Lafaye C, Signor L, Aumonier S, Flors C, Shu X, Nonell S, Gotthard G, Royant A. Sci. Rep. 9:2428 (2019)

Tracking the route of molecular oxygen in O2-tolerant membrane-bound [NiFe] hydrogenase. Kalms J, Schmidt A, Frielingsdorf S, Utesch T, Gotthard G, von Stetten D, van der Linden P, Royant A, Mroginski MA, Carpentier P, Lenz O, Scheerer P. Proc. Natl Acad. Sci. USA 115, E2229-E2237 (2018)

Lysine relay mechanism coordinates intermediate transfer in vitamin B6 biosynthesis. Rodrigues M, Windeisen V, Zhang Y, Guedez G, Weber S, Strohmeier M, Hanes J, Royant A, Evans G, Sinning I, Ealick S, Begley T, Tews I. Nat. Chem. Biol. 13, 290-294 (2017)

Precision optogenetic tool for selective single- and multiple-cell ablation in a live animal model system. Makhijani K, To T-L, Ruiz-González R, Lafaye C, Royant A, Shu X. Cell Chem. Biol. 24, 110-119 (2017)

mScarlet : a bright monomeric red fluorescent protein for cellular imaging. Bindels D S, Haarbosch L, van Weeren L, Postma M, Wiese K E, Mastop M, Aumonier S, Gotthard G, Royant A, Hink M & Gadella T W Jr (2017) Nat. Methods 14 (53-56)
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A three-dimensional movie of structural changes in bacteriorhodopsin. Nango E, Royant A, Kubo M, Nakane T, Wickstrand C, Kimura T, Tanaka T, Tono K, Song C, Tanaka R, Arima T, Yamashita A, Kobayashi J, Hosaka T, Mizohata E, Nogly P, Sugahara M, Nam D, Nomura T, Shimamura T, Im D, Fujiwara T, Yamanaka Y, Jeon B, Nishizawa T, Oda K, Fukuda M, Andersson R, Båth P, Dods R, Davidsson J, Matsuoka S, Kawatake S, Murata M, Nureki O, Owada S, Kameshima T, Hatsui T, Joti Y, Schertler G, Yabashi M, Bondar AN, Standfuss J, Neutze R, Iwata S (2016) Science ; 354(6319):1552-1557
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Structural analysis of the bright monomeric yellow-green fluorescent protein mNeonGreen obtained by directed evolution. Clavel D, Gotthard G, von Stetten D, De Sanctis D, Pasquier H, Lambert G G, Shaner N C, and Royant (2016) Acta Cryst. D 72, 1298-1307

Structural Determinants of Improved Fluorescence in a Family of Bacteriophytochrome-Based Infrared Fluorescent Proteins : Insights from Continuum Electrostatic Calculations and Molecular Dynamics Simulations. Feliks M, Lafaye C, Shu X, Royant A, Field M. (2016) Biochemistry 55 (31)

In crystallo optical spectroscopy (icOS) as a complementary tool on the macromolecular crystallography beamlines of the ESRF. von Stetten D, Giraud T, Carpentier P, Sever F, Terrien M, Dobias F, Juers D H, Flot D, Mueller-Dieckmann C, Leonard G A, de Sanctis D & Royant A (2015). Acta Cryst. D 71, 15-26

Direct evidence for a peroxide intermediate and a reactive enzyme-substrate-dioxygen configuration in a cofactor-free oxidase. Bui S, von Stetten D, Jambrina PG, Prangé T, Colloc’h N, de Sanctis D, Royant A, Rosta E, Steiner RA. (2014) Angew Chem Int Ed Engl. 53 (50).

An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Yu D, Gustafson WC, Han C, Lafaye C, Noirclerc-Savoye M, Ge W P, Thayer D A, Huang H, Kornberg T B, Royant A, Jan LY, Jan Y N, Weiss W A, Shu X. (2014) Nat Commun. 5:3626

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink M A, van Weeren L, Gadella T W Jr, Royant A. (2012) Nat Commun. 3:751
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