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Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / PETOSA Carlo

Presentation

MOLECULAR MECHANISMS OF INFECTION AND DISEASE

Team leader: Carlo Petosa

Our team is broadly interested in understanding the molecular mechanisms that underlie infectious disease. Current work focuses on:

- Nucleocytoplasmic transport of HIV proteins
- Activation of Epstein-Barr virus lytic infection
- Mass spectrometry research

The approach is to study the structure and function of key proteins involved in these processes using X-ray crystallography and complementary techniques. The long-term goal is to use the insights gained to develop new medically relevant molecules for the treatment and prevention of disease.

Key Words:


HIV, Epstein-Barr Virus, nuclear transport, lytic replication

Techniques:

- Protein expression and purification
- Biochemical and biophysical characterization of macromolecular complexes
- Molecular and cell biology
- X-ray crystallography
- Electron microscopy
- Mass spectrometry

Team members:

- Elisabetta Boeri Erba (CEA Research Engineer)
- Isabel García Sáez (CNRS Researcher)
- Rose Laure Indorato (CNRS Technician)
- Marjolaine Noirclerc-Savoye (CEA Research Engineer)
- Carlo Petosa (CNRS Research Director)
- Luca Signor (CEA Research Engineer)
- Dimitrios Skoufias (CEA Researcher)
- Kaiyao Wei (Ph.D. student)

Selected recent publications:

2018
Garcia-Saez I, Menoni H, Boopathi R, Shukla MS, Soueidan L, Noirclerc-Savoye M, Le Roy A, Skoufias DA, Bednar J, Hamiche A, Angelov D, Petosa C, Dimitrov S.
Structure of an H1-bound 6-nucleosome array reveals an untwisted two-start chromatin fiber conformation.
Mol Cell, in press.

Boeri Erba E, Signor L, Oliva MF, Hans F, Petosa C.
Characterising intact macromolecular complexes using native mass spectrometry.
Methods Mol Biol, 1764:133-151, 2018

2017
Mietton F, Ferri E, Champleboux M, Zala N, Maubon D, Zhou Y, Harbut M, Spittler D, Garnaud C, Chauvel M, d’Enfert C, Kashemirov BA, Hull M, Cornet M, McKenna CE, Govin J, Petosa C.
Selective BET bromodomain inhibition as an antifungal therapeutic strategy.
Nature Comm 8:15482, 2017

Bednar J, Garcia-Saez I, Tonchev O, Cutter A, Papai G, Reymer A, Syed SH, Lone IN, Boopathi R, Crucifix C, Menoni H, Papin C, Skoufias D, Kurumizaka H, Lavery R, Hamiche A, Hayes JJ, Schultz P, Angelov D, Petosa C, Dimitrov S.
Structure and dynamics of a 197 base-pair nucleosome in complex with linker histone H1.
Mol Cell 66:384-397, 2017

2016
Coscia F, Estrozi LF, Hans F, Malet H, Noirclerc-Savoye M, Schoehn G, Petosa C.
Fusion to a homo-oligomeric scaffold allows cryo-EM analysis of a small protein.
Sci. Reports. 6:30909. doi: 10.1038/srep30909, 2016.

Goudarzi A, Zhang D, Huang H, Barral S, Kwon O, Qi S, Tang Z, Buchou T, Vitte AL, He T, Cheng Z, Montellier E, Gaucher J, Curtet S, Debernardi A, Charbonnier G, Puthier D, Petosa P, Panne D, Rousseaux S, Roeder RG, Zhao Y, Khochbin S.
Dynamic competing histone H4 K5K8 acetylation and butyrylation are hallmarks of highly active gene promoters.
Mol Cell 62:169-180, 2016

Meyer PM, Socias S, Key J, Ransey E, Tjon E, Maia F, Buschiazzo A, Lei M, Botka C, Withrow J, Neau D, Rajashankar K, Anderson K, Baxter RH, Blacklow SC, Boggon TJ, Bonvin AMJJ, Borek D, Brett TJ, Caflisch A, Chang C-I, Chazin WJ, Corbett KD, Cosgrove MS, Crosson S, Dhe-Paganon S, Di Cera E, Drennan C, Eck M, Eichman BF, Fan QR, Ferré-D’Amaré AR, Fraser JS, Fromme JC, Garcia KC, Gaudet R, Gong P, Harrison SC, Heldwein EE, Jia Z, Keenan RJ, Kruse AC, Kvansakul M, McLellan JS, Modis Y, Otwinowski Z, Pai EF, Pereira P, Petosa C, Raman CS, Rapoport TA, Roll-Mecak A, Rosen MK, Rudenko G, Schlessinger J, Schwartz TU, Shamoo Y, Sondermann H, Tao YJ, Tolia N, Tsodikov OV, Westover KD, Wu H, Foster I, Maia FRNC, Gonen T, Kirchausen T, Diederichs K, Crosas M, Sliz P.
Data publication with the structural biology data grid supports live analysis.
Nature Comm 7:10882, 2016

Ferri E, Petosa C, McKenna CE.
Bromodomains: structure, function and pharmacology of inhibition.
Biochem Pharmacol 2016, 106:1-18.

2015
Morozumi Y, Boussouar F, Tan M, Chaikuad A, Jamshidikia M, Colak G, He H, Nie L, Petosa C, de Dieuleveult, Curtet S, Vitte AL, Schweifer N, Gianni D, Gut M, Guardiola P, Rousseaux S, Gérard M, Knapp S, Zhao Y, Khochbin S.
Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells.
J Mol Cell Biol Oct 12. pii: mjv060, 2015.

Boeri Erba E, Petosa C.
The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular assemblies.
Protein Sci. 24:1176-92, 2015