Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / PETOSA Carlo
Contact person(s) related to this article / PETOSA Carlo

Postdoc position

A postdoctoral position is available in the laboratory of Carlo Petosa at the Institute for Structural Biology (IBS) in Grenoble, France.

Project Description
Following meiosis, the mammalian genome undergoes a dramatic compaction and reorganization that involves the nearly genome-wide eviction of histones and their replacement by transition proteins and protamines. Despite its crucial importance for the mammalian life cycle, post-meiotic genome reorganization remains poorly understood. This project aims at the detailed molecular and structural characterization of the post-meiotic transitional states that occur as nucleosomes are transformed into nucleoproteins.

The postdoctoral researcher will use a biophysical and structural approach to study the transitional states that appear in post-meiotic haploid cells (for details see Barral et al., Mol Cell 2017 66:89-101). The work will include purifying endogenous macromolecular complexes from mammalian cells as well as reconstituting complexes using recombinantly expressed proteins, and subsequently using this material for biophysical analyses and for low- and high-resolution 3D structure determination, including crystallography and cryo-EM. The research will be carried out at the IBS in close collaboration with the Laboratory of Saadi Khochbin at the Institute for Advanced Biosciences.

Profile sought
Candidates should have a PhD in Biochemistry, Structural Biology or related discipline, or should expect to receive such a degree in the near future. The ideal candidate will have a strong background in biochemistry, protein purification and molecular biology and be experienced in either X-ray crystallography or cryo-EM.

Additional information
Salary on appointment will be € 2617 gross per month but may be higher depending on qualifications and experience. This is a full time post for a contract of 24 months, available 1 October 2019.

Host Institute
The IBS is a French academic research unit that currently employs 280 people and is affiliated with the University of Grenoble Alpes, the CEA and the CNRS. The institute boasts cutting-edge facilities for research in molecular and cellular biology, biochemistry, biophysics and structural biology. The IBS is a founding member of the Partnership for Structural Biology) together with three European institutes (the European Synchrotron Radiation Facility, the Institut Laue-Langevin and the EMBL Grenoble Outstation), all closely located on the European Photon and Neutron (EPN) Campus and providing a strong international environment.

Application instructions
Interested candidates are invited to submit their CV (including contact details for one or more references) and letter of motivation to carlo.petosa[at]