Présentation générale

Introduction :

L’équipe AID (Anticorps et Maladies Infectieuses), sous la direction de P. Poignard, se concentre sur l’étude de la réponse anticorps (Ab) dans les infections, avec deux domaines d’expertise principaux :

1) La caractérisation au niveau polyclonal des réponses Ab dans les infections naturelles et après vaccination, en particulier dans le contexte des infections virales. Au cours des dernières années, nous nous sommes engagés dans des projets de collaboration pour étudier les réponses Ab, notamment après la vaccination avec des candidats vaccins contre le SIDA dans des modèles animaux et dans le contexte de l’infection et de la vaccination par le SARS-CoV-2.

2) L’isolement d’anticorps monoclonaux humains par des approches basées sur des cellules B uniques, et leur caractérisation au niveau moléculaire. Cette expertise est appliquée au développement d’anticorps monoclonaux pour prévenir et traiter les maladies infectieuses. En outre, les anticorps servent d’outils pour la découverte d’antigènes de vaccins et la conception de vaccins. Au cours des dernières années, nous avons maintenu notre ligne historique d’études sur les anticorps largement neutralisants contre le VIH et étudié leur développement dans les neutralisants d’élite du VIH. En outre, nous avons mis à profit notre expertise pour nous attaquer à d’autres maladies infectieuses, en isolant des anticorps monoclonaux ciblant le virus BK, les facteurs de virulence de la bactérie multirésistante Pseudomonas aeruginosa et Plasmodium falciparum, l’agent causal du paludisme.

Thèmes et mots clés

Voie d’entrée et mécanismes de neutralisation
Rôles des anticorps neutralisants dans la prévention et le traitement de l’infection par le VIH
Anticorps neutralisants à large spectre et leur développement : coévolution Ac-glycoprotéines d’enveloppe virale

Anticorps monoclonaux humains - Neutralisation - VIH - Vaccin

Techniques spécialisées

Isolement d’anticorps monoclonaux humains
Production de protéine d’enveloppe virale (HIV / SARS-CoV-2) sous forme monomérique et trimérique
Production/Purification d’anticorps monoclonaux
Test de fixation (ELISA)
Test de neutralisation
Test pour le développement préclinique des Anticorps monoclonaux
Etude du répertoire d’anticorps par séquençage nouvelle génération

Trieur de cellules

Tutelles et financements

Publications équipe AID

2024

Target-agnostic identification of human antibodies to Plasmodium falciparum sexual forms reveals cross stage recognition of glutamate-rich repeats.
Amen A, Yoo R, Fabra-García A, Bolscher J, Stone WJR, Bally I, Dergan-Dylon S, Kucharska I, de Jong RM, de Bruijni M, Bousema T, King CR, MacGill RS, Sauerwein RW, Julien JP, Poignard P, Jore MM. eLife
https://doi.org/10.7554/eLife.97865.1

Cytomegalovirus detection is associated with ICU admission in non-AIDS and AIDS patients with Pneumocystis jirovecii pneumonia
Alexandre Perret, Marion Le Marechal, Raphaele Germi, Daniele Maubon, Cécile Garnaud, Johan Noble, Aude Boignard, Loïc Falque, Mathieu Meunier, Théophile Gerster, Olivier Epaulard
PLoS ONE, 2024, 19 (1), pp.e0296758.
https://doi.org/10.1371/journal.pon...

Development of a New Off-the-Shelf Plasmacytoid Dendritic Cell-Based Approach for the Expansion and Characterization of SARS-CoV-2-Specific T Cells
Anthony Maino, Axelle Amen, Joël Plumas, Lucie Bouquet, Marina Deschamps, Philippe Saas, Laurence Chaperot, Olivier Manches
Journal of Immunology, 2024, 212 (5), pp.825-833.
https://doi.org/10.4049/jimmunol.2300704

Results of a French external quality assessment program for Epstein Barr virus serology
Julien Lupo, Aurélie Truffot, Raphaële Germi, Anne-Karen Faure, Patrice Morand, Elizabeth Francois-Burg
Diagnostic Microbiology and Infectious Disease, 2024, 109 (3), pp.116345.
https://doi.org/10.1016/j.diagmicro...

Revisiting the interaction between complement lectin pathway protease MASP-2 and SARS-CoV-2 nucleoprotein
Isabelle Bally, Guillaume Drumont, Véronique Rossi, Serafima Guseva, Maiia Botova, Jean-Baptiste Reiser, Michel Thépaut, Sebastian Dergan Dylon, Chantal Dumestre-Pérard, Christine Gaboriaud, Franck Fieschi, Martin Blackledge, Pascal Poignard, Nicole Thielens
Frontiers in Immunology, 2024, 15, pp.1419165.
https://doi.org/10.3389/fimmu.2024....

Tixagevimab-cilgavimab (AZD7442) for the treatment of patients hospitalized with COVID-19 (DisCoVeRy): A phase 3, randomized, double-blind, placebo-controlled trial
Maya Hites, Clément Massonnaud, Eva Larranaga Lapique, Drifa Belhadi, Simon Jamard, François Goehringer, François Danion, Jean Reignier, Nathalie de Castro, Denis Garot, Karine Lacombe, Violaine Tolsma, Emmanuel Faure, Denis Malvy, Thérèse Staub, Johan Courjon, France Cazenave-Roblot, Anne Ma Dyrhol Riise, Paul Leturnier, Guillaume Martin-Blondel, Claire Roger, Karolina Akinosoglou, Vincent Le Moing, Lionel Piroth, Pierre Sellier, Xavier Lescure, Marius Trøseid, Philippe Clevenbergh, Olav Dalgard, Sébastien Gallien, Marie Gousseff, Paul Loubet, Fanny Vardon-Bounes, Clotilde Visée, Leila Belkhir, Élisabeth Botelho-Nevers, André Cabié, Anastasia Kotanidou, Fanny Lanternier, Elisabeth Rouveix-Nordon, Susana Silva, Guillaume Thiery, Pascal Poignard, Guislaine Carcelain, Alpha Diallo, Noémie Mercier, Vida Terzic, Maude Bouscambert-Duchamp, Alexandre Gaymard, Mary-Anne Trabaud, Grégory Destras, Laurence Josset, Nicolas Billard, Thi-Hong-Lien Han, Jérémie Guedj, Sandrine Couffin-Cadiergues, Aline Dechanet, Christelle Delmas, Hélène Esperou, Claire Fougerou-Leurent, Soizic Le Mestre, Anabelle Métois, Marion Noret, Isabelle Bally, Sebastián Dergan-Dylon, Sarah Tubiana, Ouifiya Kalif, Nathalie Bergaud, Benjamin Leveau, Joe Eustace, Richard Greil, Edit Hajdu, Monika Halanova, Jose-Artur Paiva, Anna Piekarska, Jesus Rodriguez Baño, Kristian Tonby, Milan Trojánek, Sotirios Tsiodras, Serhat Unal, Charles Burdet, Dominique Costagliola, Yazdan Yazdanpanah, Nathan Peiffer-Smadja, France Mentré, Florence Ader
Journal of Infection, 2024, 88 (3), pp.106120.
https://doi.org/10.1016/j.jinf.2024...

2023

Antigen pressure from two founder viruses induces multiple insertions at a single antibody position to generate broadly neutralizing HIV antibodies
Collin Joyce, Sasha Murrell, Ben Murrell, Oluwarotimi Omorodion, Lorena S. Ver, Nancy Carrico, Raiza Bastidas, Rebecca Nedellec, Michael Bick, Jordan Woehl, Fangzhu Zhao, Alison Burns, Shawn Barman, Michael Appel, Alejandra Ramos, Lalinda Wickramasinghe, Kemal Eren, Thomas Vollbrecht, Davey Smith, Sergei Kosakovsky Pond, Ryan Mcbride, Charli Worth, Facundo Batista, Devin Sok, Pascal Poignard, Bryan Briney, Ian Wilson, Elise Landais, Dennis Burton
PLoS Pathogens, 2023, 19 (6), pp.e1011416.
https://doi.org/10.1371/journal.ppa...

Heterogeneity of antibody-secreting cells infiltrating autoimmune tissues.
Giovannini D, Belbezier A, Baillet A, Bouillet L, Kawano M, Dumestre-Perard C, Clavarino G, Noble J, Pers J-O, Sturm N., Huard B
Front Immunol. 2023 Feb 21;14:1111366.
https://doi.org/10.3389/fimmu.2023....

2022

AKR1B10, One of the Triggers of Cytokine Storm in SARS-CoV2 Severe Acute Respiratory Syndrome
Clovis Chabert, Anne-Laure Vitte, Domenico Iuso, Florent Chuffart, Candice Trocme, Marlyse Buisson, Pascal Poignard, Benjamin Lardinois, Régis Debois, Sophie Rousseaux, Jean Louis Pepin, Jean Benoit Martinot, Saadi Khochbin
International Journal of Molecular Sciences, 2022, 23, pp.1911. ⟨10.3390/ijms23031911⟩
https://doi.org/10.3390/ijms23031911

Elicitation of potent SARS-CoV-2 neutralizing antibody responses through immunization with a versatile adenovirus-inspired multimerization platform
Christopher Chevillard, Axelle Amen, Solène Besson, Dalil Hannani, Isabelle Bally, Valentin Dettling, Evelyne Gout, Christophe Moreau, Marlyse Buisson, Salomé Gallet, Daphna Fenel, Emilie Vassal-Stermann, Guy Schoehn, Pascal Poignard, Marie-Claire Dagher, Pascal Fender
Molecular Therapy, 2022, 30 (5), pp.1913-1925. ⟨10.1016/j.ymthe.2022.02.011⟩
https://doi.org/10.1016/j.ymthe.202...

Fine Analysis of Lymphocyte Subpopulations in SARS-CoV-2 Infected Patients: Differential Profiling of Patients With Severe Outcome
Giovanna Clavarino, Corentin Leroy, Olivier Epaulard, Tatiana Raskovalova, Antoine Vilotitch, Martine Pernollet, Chantal Dumestre-Pérard, Federica Defendi, Marion Le Maréchal, Audrey Le Gouellec, Pierre Audoin, Jean-Luc Bosson, Pascal Poignard, Matthieu Roustit, Marie-Christine Jacob, Jean-Yves Cesbron
Frontiers in Immunology, 2022, 13, pp.889813.
https://doi.org/10.3389/fimmu.2022....

High predictive value of the soluble ZEBRA antigen (Epstein-Barr virus trans-activator Zta) in transplant patients with PTLD
Julien Lupo, Anne-Sophie Wielandts, Marlyse Buisson, Mohammed Habib, Marwan Hamoudi, Patrice Morand, Frans Verduyn-Lunel, Sophie Caillard, Emmanuel Drouet
Pathogens, 2022, 11 (8), pp.928.
https://doi.org/10.3390/pathogens11...

Human viruses, ancient, recent and zoonosis: a never ending story?
Rob Ruigrok, Emmanuel Drouet, Patrice Morand, Nicolas Tarbouriech, Rob W.H. Ruigrok
Virologie, 2022, 26 (3), pp.240-252.
https://doi.org/10.1684/vir.2022.0957

Immunization with synthetic SARS-CoV-2 S glycoprotein virus-like particles protects macaques from infection
Guidenn Sulbaran, Pauline Maisonnasse, Axelle Amen, Gregory Effantin, Delphine Guilligay, Nathalie Dereuddre-Bosquet, Judith Burger, Meliawati Poniman, Marloes Grobben, Marlyse Buisson, Sebastian Dergan Dylon, Thibaut Naninck, Julien Lemaître, Wesley Gros, Anne-Sophie Gallouët, Romain Marlin, Camille Bouillier, Vanessa Contreras, Francis Relouzat, Daphna Fenel, Michel Thepaut, Isabelle Bally, Nicole Thielens, Franck Fieschi, Guy Schoehn, Sylvie van Der Werf, Marit van Gils, Rogier Sanders, Pascal Poignard, Roger Le Grand, Winfried Weissenhorn
Cell Reports Medicine, 2022, 3 (2), pp.100528.
https://doi.org/10.1016/j.xcrm.2022...

Persistence at one year of neutralizing antibodies after SARS-CoV-2 infection: Influence of initial severity and steroid use
Olivier Epaulard, Marlyse Buisson, Benjamin Nemoz, Marion Le Maréchal, Nicolas Terzi, Jean-François Payen, Marie Froidure, Myriam Blanc, Anne-Laure Mounayar, Fanny Quénard, Isabelle Pierre, Patricia Pavese, Raphaele Germi, Laurence Grossi, Sylvie Larrat, Pascal Poignard, Julien Lupo
Journal of Infection, 2022, 84 (3), pp.418-467.
https://doi.org/10.1016/j.jinf.2021...

Potentialities of a mesoporous activated carbon as virus detection probe in aquatic systems
Doriane Delafosse, Laurence Reinert, Philippe Azaïs, Dominique Fontvieille, Yasushi Soneda, Patrice Morand, Laurent Duclaux
Journal of Virological Methods, 2022, 303, pp.114496.
https://doi.org/10.1016/j.jviromet....

Structural basis of DNA methylation-dependent site selectivity of the Epstein–Barr virus lytic switch protein ZEBRA/Zta/BZLF1
Florent Bernaudat, Montse Gustems, Johannes Günther, Mizar Oliva, Alexander Buschle, Christine Göbel, Priscilla Pagniez, Julien Lupo, Luca Signor, Christoph Müller, Patrice Morand, Michael Sattler, Wolfgang Hammerschmidt, Carlo Petosa
Nucleic Acids Research, 2022,
https://doi.org/10.1093/nar/gkab1183

2021

Clinical and laboratory characteristics of symptomatic healthcare workers with suspected COVID-19: a prospective cohort study
Antonin Bal, Karen Brengel-Pesce, Alexandre Gaymard, Grégory Quéromès, Nicolas Guibert, Emilie Frobert, Maude Bouscambert, Mary-Anne Trabaud, Florence Allantaz-Frager, Guy Oriol, Valérie Cheynet, Constance d’Aubarede, Amélie Massardier-Pilonchery, Marlyse Buisson, Julien Lupo, Bruno Pozzetto, Pascal Poignard, Bruno Lina, Jean-Baptiste Fassier, Florence Morfin, Sophie Trouillet-Assant, Jerôme Adnot, Dulce Alfaiate, Alain Bergeret, André Boibieux, Florent Bonnet, Florence Brunel-Dalmas, Eurydice Caire, Barbara Charbotel, Pierre Chiarello, Laurent Cotte, François Durupt, Vanessa Escuret, Pascal Fascia, Juliette Fontaine, Lucie Gaillot-Durand, Myriam Gillet, Matthieu Godinot, François Gueyffier, Laurence Josset, Matthieu Lahousse, Hélène Lozano, Djamila Makhloufi, Marie-Paule Milon, Frédéric Moll, David Narbey, Julie-Anne Nazare, Fatima Oria, Marielle Perry, Virginie Pitiot, Mélanie Prudent, Muriel Rabilloud, Audrey Samperiz, Isabelle Schlienger, Chantal Simon, Martine Valette
Scientific Reports, 2021, 11 (1), pp.14977.
https://doi.org/10.1038/s41598-021-...

Elicitation of potent SARS-CoV-2 neutralizing antibody responses through immunization using a versatile adenovirus-inspired multimerization platform
Christopher Chevillard, Axelle Amen, S Besson, D Hannani, Isabelle Bally, V Dettling, Evelyne Gout, Christophe J Moreau, Marlyse Buisson, Salomé Gallet, Daphna Fenel, Emilie Vassal-Stermann, Guy Schoehn, Pascal Poignard, Marie-Claire Dagher, Pascal Fender
https://doi.org/10.1016/j.ymthe.202...

Main Targets of Interest for the Development of a Prophylactic or Therapeutic Epstein-Barr Virus Vaccine
Vincent Jean-Pierre, Julien Lupo, Marlyse Buisson, Patrice Morand, Raphaële Germi
Frontiers in Microbiology, 2021, 12, pp.701611.
https://doi.org/10.3389/fmicb.2021....

Prospective evaluation of blood Epstein-Barr virus DNA load and antibody profile in HIV-related non-Hodgkin lymphomas
Julien Lupo, Raphaelle Germi, Remi Lancar, Michele Algarte-Genin, Houria Hendel-Chavez, Yassine Taoufik, Nicolas Mounier, Marialuisa Partisani, Fabrice Bonnet, Marie-Caroline Meyohas, Bruno Marchou, Anastasia Filippova, Sophie Prevot, Dominique Costagliola, Patrice Morand, Caroline Besson
AIDS. Official journal of the international AIDS Society, 2021, 35 (6), pp.861-868.
https://doi.org/10.1097/qad.0000000...

2020

Association between Neu5Gc carbohydrate and serum antibodies against it provides the molecular link to cancer: French NutriNet-Santé study
Salam Bashir, Leopold K Fezeu, Shani Leviatan Ben-Arye, Sharon Yehuda, Eliran Moshe Reuven, Fabien Szabo de Edelenyi, Imen Fellah-Hebia, Thierry Le Tourneau, Berthe Marie Imbert-Marcille, Emmanuel B Drouet, Mathilde Touvier, Jean-Christian Roussel, Hai Yu, Xi Chen, Serge Hercberg, Emanuele Cozzi, Jean-Paul Soulillou, Pilar Galan, Vered Padler-Karavani
BMC Medicine, 2020, 18 (1),
https://doi.org/10.1186/s12916-020-...

Guillain-Barré syndrome related to SARS-CoV-2 infection
Kévin Bigaut, Martial Mallaret, Seyyid Baloglu, Benjamin Nemoz, Patrice Morand, Florent Baicry, Alexandre Godon, Paul Voulleminot, Laurent Kremer, Jean-Baptiste Chanson, Jérôme de Sèze
Neurology Neuroimmunology & Neuroinflammation, 2020, 7 (5),
https://doi.org/10.1212/nxi.0000000...

Recombinant C1q variants modulate macrophage responses but do not activate the classical complement pathway
Victoria Espericueta, Ayla Manughian-Peter, Isabelle Bally, Nicole M. Thielens, Deborah Fraser
Molecular Immunology, 2020, 117, pp.65-72.
https://doi.org/10.1016/j.molimm.20...

Screening of hepatitis E in patients presenting for acute neurological disorders
Aude Belbézier, Alban Deroux, Françoise Sarrot-Reynauld, Barbara Colombe, Annick Bosseray, Claire Wintenberger, Perrine Dumanoir, Maxime Lugosi, Isabelle Boccon-Gibod, Vincent Leroy, Maxime Maignan, Roselyne Collomb-Muret, Damien Viglino, Mathieu Vaillant, Lorella Minotti, Emeline Lagrange, Olivier Epaulard, Chantal Dumestre-Perard, Sébastien Lhomme, Julien Lupo, Sylvie Larrat, Patrice Morand, Carole Schwebel, Antoine Vilotitch, Jean-Luc Bosson, Laurence Bouillet
Journal of Infection and Public Health, 2020, 13 (7), pp.1047-1050.
https://doi.org/10.1016/j.jiph.2019...

2019

Consequences of Switching to Blended Learning: The Grenoble Medical School Key Elements.
Sahal Houssein, Lionel Di Marco, Carole Schwebel, Vanda Luengo, Patrice Morand, Pierre Gillois
Studies in Health Technology and Informatics, 2019, 247, pp.356-360
PMID: 29677982

Diagnostic accuracy of a rapid RT-PCR assay for point-of-care detection of influenza A/B virus at emergency department admission: A prospective evaluation during the 2017/2018 influenza season
Maxime Maignan, Damien Viglino, Maud Hablot, Nicolas Termoz Masson, Anne Lebeugle, Roselyne Collomb Muret, Prudence Mabiala Makele, Valérie Guglielmetti, Patrice Morand, Julien Lupo, Virginie Forget, Caroline Landelle, Sylvie Larrat
PLoS ONE, 2019, 14 (5), pp.e0216308.
https://doi.org/10.1371/journal.pon...

Epstein-Barr virus biomarkers have no prognostic value in HIV-related Hodgkin lymphoma in the modern cART era
Julien Lupo, Raphaële Germi, Rémi Lancar, Michèle Algarte-Genin, Houria Hendel-Chavez, Yassine Taoufik, Nicolas Mounier, Marialuisa Partisani, Fabrice Bonnet, Caroline Meyohas, Bruno Marchou, Touyana Semenova, Sophie Prevot, Dominique Costagliola, Patrice Morand, Caroline Besson
AIDS. Official journal of the international AIDS Society, 2019, pp.1.
https://doi.org/10.1097/QAD.0000000...

Innovative multiplexed point-of-care immunoassay applied to hepatitis B screening
S Delshadi, G Blaire, V Massé, T Decaens, Sylvie Larrat, Patrice Morand, O Cugat, Pn Marche, P Kauffmann
Clinica Chimica Acta, 2019, 493, pp.S644—S645.
https://doi.org/10.1016/j.cca.2019....

Using high-throughput sequencing for investigating intra-host hepatitis C evolution over long retrospective periods
A. Caporossi, O. Kulkarni, M. Blum, V. Leroy, Patrice Morand, Sylvie Larrat, O. François
Infection, Genetics and Evolution, 2019, 67, pp.136-144.
https://doi.org/10.1016/j.meegid.20...

Utility of Epstein-Barr Virus Biomarkers in Human Immunodeficiency Virus–related Lymphomas in the Modern Combined Antiretroviral Therapy Era
Julien Lupo, Raphaële Germi, Dominique Costagliola, Patrice Morand, Caroline Besson
Clinical Infectious Diseases, 2019, 68 (5), pp.891-892.
https://doi.org/10.1093/cid/ciy786

2018

Emerging resistance mutations in PI-naive patients failing an atazanavir-based regimen (ANRS multicentre observational study)
L Lambert-Niclot, C Grude, L. Chaix, L Charpentier, C Reigadas, L Le Guillou-Guillemette, C Rodallec, Pascale Maillard, C Dufayard, C Mourez, C Mirand, L Guinard, S Montes, L Vallet, Marc Marcelin, L Descamps, C Flandre, L Delaugerre, Chakib Alloui, Diane Descamps, Charlotte Charpentier, Benoit Visseaux, Anne Krivine, Ali Bouviers-Alias, Coralie Pallier, Cathia Soulié, Marc Wirden, Anne Marcelin, Vincent Calvez, Laurence Morand, Sidonie Lambert-Niclot, Djeneba Fofana, Nadia Mahjoub, Constance Delaugerre, Marie Chaix, Corinne Amiel, Veronique Schneider, Catherine Roussel, Hélène Le Guillou-Guillemette, Laurence Courdavault, Sandrine Reigadas, Patricia Recordon-Pinson, Hervé Fleury, Sophie Vallet, Julia Dina, Astrid Vabret, Audrey Mirand, Cécile Henquell, Christelle Auvray, Alexis de Rougemont, Helene Giraudon, Ali Si-Mohammed, Dominique Mathez, Anne Signori-Schmuck, Patrice Morand, Laurence Bocket, Anne Trabaud, Brigitte Montes, Laura Le Guen, Audrey Rodallec, Virginie Ferré, Hélène Jeulin, Evelyne Schvoerer, Jacqueline Dufayard, Annick Allardet-Servent, Marie Carles, Jérôme Guinard, Aurélie Guigon, Genevieve Giraudeau, Agnès Beby-Defaux, Anne Maillard, Jean Christophe Plantier, Marie Leoz, Thomas Mourez, Thomas Bourlet, Samira Fafi-Kremer, Julie Chiabrando, Stéphanie Raymond, Jacques Izopet, Francis Barin, Stéphanie Marque-Juillet, Sabine Yerly
Journal of Antimicrobial Chemotherapy, 2018, 73 (8), pp.2147 - 2151.
https://doi.org/10.1093/jac/dky142

Evaluation of the cobas® GT hepatitis C virus genotyping assay in G1-6 viruses including low viral loads and LiPA failures
Benjamin Némoz, Léa Roger, Vincent Leroy, Jean-Dominique Poveda, Patrice Morand, Sylvie Larrat
PLoS ONE, 2018, 13 (3), pp.e0194396.
https://doi.org/10.1371/journal.pon...

Exogenous human herpesvirus 6 reinfection after tumor-infiltrating T-lymphocyte therapy
Anastasiia Filippova, Julie Charles, Olivier Epaulard, Raphaële Germi, Virginie Persoons, Isabelle Templier, Marie-Thérèse Leccia, Brigitte Dreno, Irina Malova, Patrice Morand, Julien Lupo
Cytotherapy, 2018, 20 (4), pp.521-523.
https://doi.org/10.1016/j.jcyt.2017...

Misidentification of recombinant hepatitis C virus leading to treatment failure with direct acting antivirals
Thomas Mourez, Amandine Decroos, Odile Goria, Hélène Montialoux, Fabienne de Oliveira, Sylvie Larrat, Jean-Christophe Plantier, Ghassan Riachi
Journal of Medical Virology, 2018, 90 (5), pp.994-997.
https://doi.org/10.1002/jmv.25035

ReSynPlex: Respiratory Syndrome Linked Pathogens Multiplex Detection and Characterization
L. Villiers, Y. Caspar, H. Marche, S. Boccoz, M. Maurin, P.N. Marche, Patrice Morand, C.A. Marquette, B.P. Corgier
Innovation and Research in BioMedical engineering, 2018, 39 (5), pp.368-375. ⟨10.1016/j.irbm.2018.10.002⟩
https://inserm.hal.science/inserm-02332411/file/Villiers%20et%20al_IRBM-2018.pdf BibTex
accès restreint

2017

Added Value of Next-Generation Sequencing for Multilocus Sequence Typing Analysis of a Pneumocystis jirovecii Pneumonia Outbreak1
Eléna Charpentier, Cécile Garnaud, Claire Wintenberger, Sébastien Bailly, Jean-Benjamin Murat, John Rendu, Patricia Pavese, Thibault Drouet, Caroline Augier, Paolo Malvezzi, Anne Thiebaut-Bertrand, Marie-Reine Mallaret, Olivier Epaulard, Muriel Cornet, Sylvie Larrat, Danièle Maubon
Emerging Infectious Diseases, 2017, 23 (8), pp.1237 - 1245.
https://doi.org/10.3201/eid2308.161295

An ancestral retroviral protein identified as a therapeutic target in type-1 diabetes
Sandrine Levet, Julie Medina, Julie Joanou, Amandine Demolder, Nelly Queruel, Kevin Réant, Matthieu Normand, Marine Seffals, Julie Dimier, Raphaële Germi, Thomas Piofczyk, Jacques Portoukalian, Jean-Louis Touraine, Hervé Perron
JCI Insight, 2017, 2 (17),
https://doi.org/10.1172/jci.insight...

Endogenous retroviral sequences in the human genome can play a physiological or pathological role
Julie Medina, Benjamin Charvet, Pascal Leblanc, Raphaële Germi, Branka Horvat, Patrice N. Marche, Hervé Perron
Médecine/Sciences, 2017, 33 (4), pp.397-403.
https://doi.org/10.1051/medsci/2017...

Epidemiology of infectious encephalitis causes in 2016
A. Boucher, J.L. Herrmann, Patrice Morand, R. Buzelé, A Crabol, A Stahl, A. Mailles
Médecine et Maladies Infectieuses, 2017, 47 (3), pp.221-235.
https://doi.org/10.1016/j.medmal.20...

Evolution of EBV seroprevalence and primary infection age in a French hospital and a city laboratory network, 2000–2016
Grégoire Fourcade, Raphaële Germi, Fabrice Guerber, Julien Lupo, Monique Baccard, Arnaud Seigneurin, Touyana Semenova, Patrice Morand, Olivier Epaulard
PLoS ONE, 2017, 12 (4), pp.e0175574.
https://doi.org/10.1371/journal.pon...

Fatal autochthonous fulminant hepatitis E early after allogeneic stem cell transplantation
M. Carre, A Thiebaut-Bertrand, Sylvie Larrat, V Leroy, P. Pouzol, N Sturm, S Lhomme, J-y Cahn, F. Garban, Patrice Morand
Bone Marrow Transplantation, 2017, 52 (4), pp.643-645.
https://doi.org/10.1038/bmt.2016.337

Hepatitis E Virus Infection after Platelet Transfusion in an Immunocompetent Trauma Patient
Emmanuelle Loyrion, Thibaut Trouve-Buisson, Patricia Pouzol, Sylvie Larrat, Thomas Decaens, Jean-François Payen
Emerging Infectious Diseases, 2017, 23 (1), pp.146-147.
https://doi.org/10.3201/eid2301.160923

Herpes simplex type 2 encephalitis and methotrexate medication: a fortuitous or causative association in a patient with spondyloarthritis?
Julien Lupo, Ophélie dos Santos, Raphaële Germi, Monique Baccard-Longère, Jean-Paul Stahl, Olivier Epaulard, Patrice Morand
Antiviral Therapy, 2017, 22 (4), pp.357-359.
https://doi.org/10.3851/imp3110

HIV Envelope Glycoform Heterogeneity and Localized Diversity Govern the Initiation and Maturation of a V2 Apex Broadly Neutralizing Antibody Lineage
Elise Landais, Ben Murrell, Bryan Briney, Sasha Murrell, Kimmo Rantalainen, Zachary T Berndsen, Alejandra Ramos, Lalinda Wickramasinghe, Melissa Laird Smith, Kemal Eren, Natalia de Val, Mengyu Wu, Audrey Cappelletti, Jeffrey Umotoy, Yolanda Lie, Terri Wrin, Paul Algate, Po-Ying Chan-Hui, Etienne Karita, Andrew B Ward, Ian A Wilson, Dennis R Burton, Davey Smith, Sergei L Kosakovsky Pond, Pascal Poignard
Immunity, 2017, 47 (5), pp.990 - 1003.e9.
https://doi.org/10.1016/j.immuni.20...

Lytic EBV infection investigated by detection of Soluble Epstein-Barr virus ZEBRA in the serum of patients with PTLD
Mohammed Habib, Marlyse Buisson, Julien Lupo, Félix Agbalika, Gérard Socié, Raphaële Germi, Monique Baccard, Berthe-Marie Imbert-Marcille, Jacques Dantal, Patrice Morand, Emmanuel Drouet
Scientific Reports, 2017, 7 (1), pp.10479.
https://doi.org/10.1038/s41598-017-...

Plasma Circulating Tumor DNA Levels for the Monitoring of Melanoma Patients: Landscape of Available Technologies and Clinical Applications
Benoit Busser, Julien Lupo, Lucie Sancey, Stéphane Mouret, Patrice Faure, Joël Plumas, Laurence Chaperot, Marie Therese Leccia, Jean Luc Coll, Amandine Hurbin, Pierre Hainaut, Julie Charles
BioMed Research International , 2017, 2017, pp.5986129.
https://doi.org/10.1155/2017/5986129

Ultradeep sequencing of B and non-B HIV-1 subtypes: Viral diversity and drug resistance mutations before and after one month of antiretroviral therapy in naive patients
Olivier Epaulard, Anne Signori-Schmuck, Sylvie Larrat, Om Kulkarni, Michael Blum, Katia Fusillier, Myriam Blanc, Pascale Leclercq, Olivier François, Patrice Morand
Journal of Clinical Virology, 2017, 95, pp.13-19
https://doi.org/10.1016/j.jcv.2017....

Virus-driven Inflammation Is Associated With the Development of bNAbs in Spontaneous Controllers of HIV.
Anne-Sophie Dugast, Kelly Arnold, Giuseppe Lofano, Sarah Moore, Michelle Hoffner, Melissa Simek, Pascal Poignard, Michael Seaman, Todd J Suscovich, Florencia Pereyra, Bruce D Walker, Doug Lauffenburger, Douglas S Kwon, Brandon F Keele, Galit Alter
Clinical Infectious Diseases, 2017, 64 (8), pp.1098-1104.
https://doi.org/10.1093/cid/cix057

2016

A Prominent Site of Antibody Vulnerability on HIV Envelope Incorporates a Motif Associated with CCR5 Binding and Its Camouflaging Glycans.
Devin Sok, Matthias Pauthner, Bryan Briney, Jeong Hyun Le, Karen L Saye-Francisco, Jessica Hsueh, Alejandra Ramos, Khoa M Le, Meaghan Jones, Joseph G Jardine, Raiza Bastidas, Anita Sarkar, Chi-Hui Liang, Sachin S Shivatare, Chung-Yi Wu, William R Schief, Chi-Huey Wong, Ian A Wilson, Andrew B Ward, Jiang Zhu, Pascal Poignard, Dennis R Burton
Immunity, 2016, 45 (1), pp.31-45.
https://doi.org/10.1016/j.immuni.20...

Amplification and pyrosequencing of near-full-length hepatitis C virus for typing and Monitoring antiviral Resistant Strains
Pauline Trémeaux, Alban Caporossi, Christophe Ramière, Elodie Santoni, Nicolas Tarbouriech, Marie-Ange Thélu, Katia Fusillier, Laure Geneletti, Olivier François, Vincent Leroy, Wim P Burmeister, Patrice André, Patrice Morand, Sylvie Larrat
Clinical Microbiology and Infection, 2016,
https://doi.org/10.1016/j.cmi.2016....

Broadly Neutralizing Antibody Responses in a Large Longitudinal Sub-Saharan HIV Primary Infection Cohort
Elise Landais, Xiayu Huang, Colin Havenar-Daughton, Ben Murrell, Matt A. Price, Lalinda Wickramasinghe, Alejandra Ramos, Charoan B. Bian, Melissa Simek, Susan Allen, Etienne Karita, William Kilembe, Shabir Lakhi, Mubiana Inambao, Anatoli Kamali, Eduard J. Sanders, Omu Anzala, Vinodh Edward, Linda-Gail Bekker, Jianming Tang, Jill Gilmour, Sergei L. Kosakovsky-Pond, Pham Phung, Terri Wrin, Shane Crotty, Adam Godzik, Pascal Poignard
PLoS Pathogens, 2016, 12 (1),
https://doi.org/10.1371/journal.ppa...

CXCL13 is a plasma biomarker of germinal center activity.
Colin Havenar-Daughton, Madelene Lindqvist, Antje Heit, Jennifer E Wu, Samantha M Reiss, Kayla Kendric, Simon Bélanger, Sudhir Pai Kasturi, Elise Landais, Rama S Akondy, Helen M Mcguire, Marcella Bothwell, Parsia A Vagefi, Eileen Scully, Georgia D Tomaras, Mark M Davis, Pascal Poignard, Rafi Ahmed, Bruce D Walker, Bali Pulendran, M Juliana Mcelrath, Daniel E Kaufmann, Shane Crotty
Proceedings of the National Academy of Sciences of the United States of America, 2016, 113 (10), pp.2702-7.
https://doi.org/10.1073/pnas.1520112113

Different precore/core mutations of hepatitis B interact with, limit, or favor liver fibrosis severity
Alexandra Ducancelle, Adeline Pivert, Sandrine Bertrais, Jérôme Boursier, Viorica Balan, Pascal Veillon, Hélène Le Guillou-Guillemette, Vincent Thibault, Sandrine Castelain, Bénédicte Roquebert, Marianne Coste-Burel, Vincent Mackiewicz, Evelyne Schvoerer, Sylvie Larrat, Sarah Maylin, Sophie Alain, Véronique Loustaud-Ratti, Emmanuel Gordien, Joël Gozlan, Véronique Brodard, Stéphane Chevaliez, Paul Calès, Françoise Lunel-Fabiani
Journal of Gastroenterology and Hepatology, 2016, 31 (10), pp.1750 - 1756.
https://doi.org/10.1111/jgh.13338

Dynamics of Epstein-Barr viral load after hematopoietic stem cell transplantation and effect of preemptive rituximab therapy
Mihaja Raberahona, Chloe Wackenheim, Raphaële Germi, Martin Carré, Claude-Eric Bulabois, Anne C.M. Thiébaut, Julien Lupo, Touyana Semenova, Jean-Yves Cahn, Patrice Morand, Olivier Epaulard
Transplant Infectious Disease, 2016, 18 (6), pp.889-895.
https://doi.org/10.1111/tid.12618

Early Antibody Lineage Diversification and Independent Limb Maturation Lead to Broad HIV-1 Neutralization Targeting the Env High-Mannose Patch.
Daniel T Macleod, Nancy M Choi, Bryan Briney, Fernando Garces, Lorena S Ver, Elise Landais, Ben Murrell, Terri Wrin, William Kilembe, Chi-Hui Liang, Alejandra Ramos, Chaoran B Bian, Lalinda Wickramasinghe, Leopold Kong, Kemal Eren, Chung-Yi Wu, Chi-Huey Wong, Sergei L Kosakovsky Pond, Ian A Wilson, Dennis R Burton, Pascal Poignard
Immunity, 2016, 44 (5), pp.1215-26.
https://doi.org/10.1016/j.immuni.20...

Elaboration of a clinical and paraclinical score to estimate the probability of herpes simplex virus encephalitis in patients with febrile, acute neurologic impairment
S. Gennai, A. Rallo, D. Keil, A. Seigneurin, Raphaële Germi, O. Epaulard
European Journal of Clinical Microbiology and Infectious Diseases, 2016, 35 (6), pp.935-939.
https://doi.org/10.1007/s10096-016-...

Ensemble Structure of the Highly Flexible Complex Formed between Vesicular Stomatitis Virus Unassembled Nucleoprotein and its Phosphoprotein Chaperone.
Filip Yabukarski, Cedric Leyrat, Nicolas Martinez, Guillaume Communie, Ivan Ivanov, Euripedes A Ribeiro, Marlyse Buisson, Francine Gérard, Jean-Marie Bourhis, Malene Ringkjøbing Jensen, Pau Bernadó, Martin Blackledge, Marc Jamin
Journal of Molecular Biology, 2016, 428 (13), pp.2671-94.
https://doi.org/10.1016/j.jmb.2016....

Fatal case of acute gastroenteritis with multiple viral coinfections.
Julien Lupo, Christine Morel-Baccard, Anne-Pascale Michard-Lenoir, Raphaële Germi, Pierre Pothier, Katia Ambert-Balay, Patrice Morand
Journal of Clinical Virology, 2016, 74, pp.54-56.
https://doi.org/10.1016/j.jcv.2015....

First next-generation sequencing full-genome characterization of a hepatitis C virus genotype 7 divergent subtype
M. Salmona, A. Caporossi, P. Simmonds, M. Thélu, K. Fusillier, S. Mercier-Delarue, N. de Castro, J. Legoff, M. Chaix, O. François, F. Simon, Patrice Morand, Sylvie Larrat, S. Maylin
Clinical Microbiology and Infection, 2016, 22 (11), pp.947.e1-947.e8
https://doi.org/10.1016/j.cmi.2016....

Hepatitis C virus whole genome sequencing: Current methods/issues and future challenges
Pauline Trémeaux, Alban Caporossi, Marie-Ange Thélu, Michael Blum, Vincent Leroy, Patrice Morand, Sylvie Larrat
Critical Reviews in Clinical Laboratory Sciences, 2016, 53 (5), pp.341-351.
https://doi.org/10.3109/10408363.20...

Methylation of Epstein-Barr virus Rta promoter in EBV primary infection, reactivation and lymphoproliferation
Raphaële Germi, Nicolas Guigue, Julien Lupo, Touyana Semenova, Laurence Grossi, Odile Vermeulen, Olivier Epaulard, Florence de Fraipont, Patrice Morand
Journal of Medical Virology, 2016, 88 (10), pp.1814-1820.
https://doi.org/10.1002/jmv.24524

Multicenter Evaluation of Whole-Blood Epstein-Barr Viral Load Standardization Using the WHO International Standard
Touyana Semenova, Julien Lupo, Sophie Alain, Gwladys Perrin-Confort, Laurence Grossi, Julie Dimier, Olivier Epaulard, Patrice Morand, Raphaële Germi
Journal of Clinical Microbiology, 2016, 54 (7), pp.1746-1750.
https://doi.org/10.1128/jcm.03336-15

Antibodies to a conformational epitope on gp41 neutralize HIV-1 by destabilizing the Env spike.
Jeong Hyun Lee, Daniel P Leaman, Arthur S Kim, Alba Torrents de La Peña, Kwinten Sliepen, Anila Yasmeen, Ronald Derking, Alejandra Ramos, Steven W de Taeye, Gabriel Ozorowski, Florian Klein, Dennis R Burton, Michel C Nussenzweig, Pascal Poignard, John P Moore, Per Johan Klasse, Rogier W Sanders, Michael B Zwick, Ian A Wilson, Andrew B Ward
Nature Communications, 2015, 6, pp.8167.
https://doi.org/10.1038/ncomms9167

Identification of Common Features in Prototype Broadly Neutralizing Antibodies to HIV Envelope V2 Apex to Facilitate Vaccine Design.
Raiees Andrabi, James E Voss, Chi-Hui Liang, Bryan Briney, Laura E Mccoy, Chung-Yi Wu, Chi-Huey Wong, Pascal Poignard, Dennis R Burton
Immunity, 2015, 43 (5), pp.959-73.
https://doi.org/10.1016/j.immuni.20...

Mechanisms of escape from the PGT128 family of anti-HIV broadly neutralizing antibodies.
Stefanie A Krumm, Hajer Mohammed, Khoa M Le, Max Crispin, Terri Wrin, Pascal Poignard, Dennis R Burton, Katie J Doores
Retrovirology, 2015, 13, pp.8.
https://doi.org/10.1186/s12977-016-...