Combining X-ray crystallography, EPR spectroscopy and theoretical calculations, researchers from IBS-METALLO and INAC-CAMPS have determined the fine mechanism for carboxyl radical migration during methylindolic acid biosynthesis. A preorganized active site and restrained motions are essential to afford an efficient reaction.
Radical S-Adenosyl-l-methionine Tryptophan Lyase (NosL) : How the Protein Controls the Carboxyl Radical •CO2- Migration. Amara P, Mouesca JM, Bella M, Martin L, Saragaglia C, Gambarelli S, Nicolet Y. J Am Chem Soc. 2018 Nov 16. doi : 10.1021/jacs.8b09142