Séminaire IBS : Understanding tissue by volumeEM – some examples from an EM platform

Par Dr Christel Genoud (Université de Lausanne & EPFL, Suisse)

Understanding the complex architecture of cells and tissues requires imaging technologies
that can bridge the gap between ultrastructural details and large-volume context in room temperature and cryo-EM. Volume electron microscopy (volume EM) addresses this need by enabling 3D imaging of biological
samples at nanometer resolution over tens to hundreds of microns. In this presentation, I will provide an overview of volume EM techniques available on our platform based on examples and how we combine it with correlative light and electron microscopy.
I will also show how we are adressing the targeting of small structures in lare tissue in cryo-ET by using the serial lift-out method and cryo-CLEM. The example of targeting the Casparian strip in the root of Arabidopsis thaliana will be developed.

Hôte : Lefeteris Zarkadas (IBS/MEM)