Séminaire IBS : Unraveling virus replication-induced organelle remodelling by cellular cryo-electron tomography


Salle des séminaire IBS

Par Dr Petr Chlanda (Université de Heidelberg Allemagne)

Viruses developed various strategies to remodel infected cells to carry out genome replication and generate new progeny. While positive single-stranded RNA viruses hijack membranous organelles to form replication vesicles, many negative single-stranded RNA viruses use liquid organelles as a site of viral genome replication. We apply cellular cryo-electron tomography and correlative light and electron microscopy to study the replication and assembly of SARS-CoV-2 and Ebola viruses. We show that SARS-CoV-2 nsp3-4 are minimal components of the pore spanning double-membrane vesicles. In addition, we revealed Ebola virus nucleocapsid assembly inside membrane-less inclusion bodies using subtomogram averaging.

Hôte : Irina Gutsche (IBS/Groupe Imagerie microscopique d’assemblages complexes)