Research Project
We are interested in studying the regulatory mechanisms of base excision repair (BER) within the nucleus. More specifically, we use human cell line models to : (i) characterize, by mass spectrometry, the interaction partners and post-translational modifications (PTMs) of repair factors involved in BER, and (ii) analyze their distribution and mobility using single-molecule localization microscopy (SMLM) super-resolution imaging.
These combined approaches will allow us to gain a better understanding of the spatial and dynamic organization of BER players in the nucleus, to elucidate the regulatory mechanisms that determine its efficiency, and to identify new factors contributing to the cellular response to oxidative or alkylating damage.
Team members
. Elise Pouponnot (PhD student)
. Mrinalini Lianne Rao (PhD student)
. Pierre Caron (CRCN, CNRS)
. Fabienne Hans (Enseignante-chercheuse UGA)
Plateform
. IBS M4D Imaging Platform
Collaborations
. Yohann Couté - EDyP Lab - CEA-IRIG, Grenoble, France
Publications