Anaerobic conditions freezing grids

In collaboration with the Metalloprotein group at IBS, we have developed a grid freezing method for cryo-ME under anaerobic conditions. A Vitrobot was put in a glove box and several samples were frozen under these conditions as a proof of principle.

To use this system, please contact Guy Schoehn or Yvain Nicolet.

Publication : Mickaël V Cherrier, Xavier Vernède, Daphna Fenel, Lydie Martin, Benoit Arragain, Emmanuelle Neumann, Juan C Fontecilla-Camps, Guy Schoehn, Yvain Nicolet. Oxygen-Sensitive Metalloprotein Structure Determination by Cryo-Electron Microscopy . Biomolecules, 2022 Mar 12;12(3):441.
doi: 10.3390/biom12030441