Accueil > Research > Research groups > Biomolecular NMR Spectroscopy Group (Bernhard Brutscher) > Team NMR of large Biomolecular Assemblies
NMR of Large Biomolecular AssembliesTeam MembersJérôme Boisbouvier (CNRS Researcher) If you want to contact team members, please use the generic e-mail address : firstname(dot)lastname(at)ibs(dot)fr PresentationOur team is synergistically developing advanced in vivo and in vitro labeling methods together with optimized NMR spectroscopy to push forward the limit of application of NMR into Biology. We are integrating these innovative methods with other biophysics approaches in order to study folding, structure, dynamics, transient interactions and mechanism of essential cellular machineries and large chaperones Recent Applications :
Self-assembling of the 468kDa TET2 proteolytic machine.
Structure determination of a 0.5 MDa complex using NMR and cryo-EM.
Structural and Functional Study of a 1 MDa Chaperonin in Action.
The team is operating the ISBG Cell-Free Facility offering the possibility to visiting scientists to express in large scale target proteins for structural biology investigation. For more information visit cell-free facility page here Major Publications :• Van Melckebeke H, Devany M, Di Primo C, Beaurain F, Toulmé JJ, Bryce DL, Boisbouvier J. “Liquid Crystal NMR Structure of HIV TAR RNA Bound to its SELEX RNA Aptamer Reveals the Origins of the High Stability of the Complex“ Proc. Natl. Acad. Sci. USA 105, 9210-5 (2008). • Amero C, Schanda P, Dura A, Ayala I, Marion D, Franzetti B, Brutscher B, Boisbouvier J. “Fast Two Dimensional NMR Spectroscopy of High Molecular Weight Protein Assemblies”. J. Am. Chem. Soc. 131, 3448-9 (2009). • Gans P, Hamelin O, Sounier R, Ayala I, Dura MA, Amero C, Noirclerc-Savoye M, Franzetti B, Plevin MJ, Boisbouvier J. “Stereospecific Isotopic Labeling of Methyl Groups for NMR Studies of High Molecular Weight Proteins” Angew. Chem. Int. Ed. 49, 1958-1862 (2010). • PlevinMJ, Bryce DL, Boisbouvier J. “Direct Detection of CH••Pi Interactions in Proteins” Nature Chemistry. 2, 466-471 (2010). • Kerfah R, Plevin MJ, Sounier R., Gans P, Boisbouvier J. “Methyl Specific Isotopic Labeling : A Molecular Tool Box for NMR Studies of Large Proteins“ Current Opinion in Structural Biology 32, 113-122 (2015). • Macek P, Kerfah R, Boeri Erba E, Crublet E, Moriscot C, Schoehn G, Amero C, Boisbouvier J. “Unraveling Self-Assembly Pathways of the 468 kDa Proteolytic Machine TET2” Science Advances 3, e1601601 (2017). • Mas G, Guan J-Y, Crublet E, Colas Debled E, Moriscot C, Gans P, Schoehn G, Macek P, Schanda P, Boisbouvier J. “Structural Investigation of a Chaperonin in Action Reveals How Nucleotide Binding Regulates the Functional Cycle“ Science Advances 4, eaau4196 (2018). • Gauto D, Estrozi L, Schwieters C, Effantin G, Macek P, Sounier R, Sivertsen AC, Schmidt E, Kerfah R, Mas G, Colletier JP, Güntert P, Favier A, Schoehn G, Schanda P, Boisbouvier J. “Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex“ Nature Communication 10(1):2697 (2019). Collaborations :ForschungsZentrum-Jülich (L. Gremer ; W. Hoyer ; D. Willbold) ; HITS-Heildelberg (D. Kokh ; K. Sadiq ; R. Wade) ; IBS (B. Brutscher ; P. Schanda ; G. Schoehn) ; ILM-Lyon (C. Loison) ; Sanofi (O. Frances) ; University of Lorraine (C. Chipot, F. Dehez) ; University P. & M. Curie - Paris (E. Miclet). Funding Agencies :
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