Accueil > Research > Research groups > Dynamics and kinetics of molecular processes Group (M.Weik) > SNAX
PresentationSerial nanocrystallography(PI Colletier) Research summery Members Collaborations Research activity Our motivation to obtain nanocrystals of OCP is thus two-fold. First, a success with the in vivo crystallisation of OCP would demonstrate the reaches of recombinant nanocrystallization in Bt, establishing that not only an exogenous protein – such as BinAB – but also its pigment can be recombinantly co-produced and co-crystallised. Our long-term goal is indeed to establish in vivo nanocrystallisation as a method of choice for investigators seeking to produce high amount of nanocrystalline material (e.g. TR-SFX), or to solve structures of fragile protein and protein-complexes which — alike BinAB — do not easily crystallize and/or degrade following extraction from the cell. Second, the SNaX team works in collaboration with the groups of Diana Kirilovsky (IB2C, Saclay), Michel Sliwa (Lasir, Lilles) and Ilme Schlichting (Max Planck Institute for Biomedical Research, Heidelberg, Germany) towards developing more active OCP variants to be used as regulators of light uptake in artificial photosynthetic systems. Key publications Nass Kovacs G, Colletier JP, Grünbein ML, Yang Y, Stensitzki T, Batyuk A, Carbajo S, Doak RB, Ehrenberg D, Foucar L, Gasper R, Gorel A, Hilpert M, Kloos M, Koglin JE, Reinstein J, Roome CM, Schlesinger R, Seaberg M, Shoeman RL, Stricker M, Boutet S, Haacke S, Heberle J, Heyne K, Domratcheva T1, Barends TRM & Schlichting I (2019) Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin Nat. Comm. 10 : 3177 El-Khatib M, Nasrallah C, Lopes J, Tran QT, Tetreau G, Basbous H, Fenel D, Gallet B, Lethier M, Bolla JM, Pages JM, Vivaudou M, Weik M, Winterhalter M, Colletier JP (2018) Porin self-association enables cell-to-cell contact in Providencia stuartii floating communities. Proc Natl Acad Sci U S A 115 : E2220-E2228 Tayeb-Fligelman E, Tabachnikov O, Moshe M, Goldshmidt-Tran O, Sawaya MR, Coquelle N, Colletier J-P, Landau M (2016) The Cytotoxic Staphylococcus aureus PSMα3 Reveals a Novel Cross-alpha Amyloid-like Fibril. Science 355 : 831-833. Colletier JP, Sawaya MR, Gingery M, Rodriguez JA, Cascio D, Brewster AS, Michels-Clark T, Hice RH, Coquelle N, Boutet S, Williams GJ, Messerschmidt M, DePonte DP, Sierra RG, Laksmono H, Koglin JE, Hunter MS, Park HW, Uervirojnangkoorn M, Bideshi DK, Brunger AT, Federici BA, Sauter NK, Eisenberg DS. (2016) A potent binary mosquito larvicide revealed by de novo phasing with an X-ray free-electron laser. Nature 539 : 43-47. Coquelle N, Brewster AS, Kapp U, Shilova A, Weinhausen B, Burghammer M, Colletier J-P (2015) Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams. Acta Crystallogr D Biol Crystallogr 71 : 1184–1196. Tetreau G, Banneville AS, Andreeva E, Brewster AS, Hunter M, Sierra R, Teulon JM, Young I, Burke N, Grünewald T, Beaudouin J, Snigireva I, Fernandez-Luna MT, Burt A, Park HW, Signor L, Bafna J, Sadir R, Fenel D, Boeri-Erba E, Bacia M, Zala N, Laporte F, Després L, Weik M, Boutet S, Rosenthal M, Coquelle N, Burghammer M, Cascio D, Sawaya M, Winterhalter M, Gratton E, Gutsche I, Federici B, Pellequer JL, Sauter N, Colletier JP (2020) De la Mora E, Coquelle N, Bury C, Rosenthal R, Holton J, Carmichael I, Garman E, Burghammer M, Colletier JP, Weik M (2020) |