PresentationGroup leader : Winfried Weissenhorn MembersPermanent Non-permanent Research topicsThe focus of our research is to obtain insight into the structural and functional role of proteins and complexes that control and catalyze entry and budding processes of enveloped viruses such as HIV-1. We employ a combination of molecular biology, protein chemistry, X-ray crystallography and electron microscopy (collaboration) to address these questions. Enveloped virus budding
Model for ESCRT-III driven HIV-1 budding HIV-1 entry KeywordsHIV-1, Env, gp41, bnAbs, ESCRT, membrane fission, budding, X-ray crystallography. Selected publications• Caillat C, Guilligay D, Torralba J, Friedrich N, Nieva JL, Trkola A, Chipot CJ, Dehez FL, Weissenhorn W. (2021) Structure of HIV-1 gp41 with its membrane anchors targeted by neutralizing antibodies. Elife, 10:e65005. doi : 10.7554/eLife.65005. • Bertin A, de Franceschi N, de la Mora E, Maity S, Alqabandi M, Miguet N, di Cicco A, Roos W, Mangenot S, Weissenhorn W and Bassereau P, (2020) Human ESCRT-III Polymers Assemble on Positively Curved Membranes and Induce Helical Membrane Tube Formation. Nat Commun, 11(1):2663. doi : 10.1038/s41467-020-16368-5. • Pinto, D., Fenwick, C., Caillat, C., Silacci, C., Guseva, S., Dehez, F., Chipot, C., Barbieri, S., Minola, A., Jarrossay, D., Tomaras, G.D., Shen, X., Riva, A., Tarkowski, M., Schwartz, O., Bruel, T., Dufloo, J., Seaman, M.S., Montefiori, D.C., Lanzavecchia, A., Corti, D., Pantaleo, G. and Weissenhorn, W. (2019) Structural Basis for Broad HIV-1 Neutralization by the MPER-Specific Human Broadly Neutralizing Antibody LN01. Cell Host & Microbe, 26(5):623-637.e8. doi : 10.1016/j.chom.2019.09.016. • Maity S, Caillat C, Miguet N, Sulbaran G, Effantin G, Schoehn G, Roos WH, & Weissenhorn W (2019) VPS4 triggers constriction and cleavage of ESCRT-III helical filaments. Sci Adv 5(4) : eaau7198.doi : 10.1126/sciadv.aau7198.. • Ventimiglia, L.N., Cuesta-Geijo, M. A., Martinelli, N., Caballe, A., Macheboeuf, P., Miguet, M., Parnham, I. M., Olmos, Y., Carlton, J. G., Weissenhorn W. and Martin-Serrano, J. (2018) CC2D1B coordinates ESCRT-III activity during the mitotic reformation of the nuclear envelope. Dev Cell 47, 547-563. doi : 10.1016/j.devcel.2018.11.012. • Crespo-Yàñez X, Aguilar-Gurrieri C, Jacomin AC, Journet A, Mortier M, Taillebourg E, Soleilhac E, Weissenhorn W and Fauvarque MO. (2018) CHMP1B is a target of USP8/UBPY regulated by ubiquitin during endocytosis. PLoS Genet. 14(6):e1007456. • Effantin, G., Estrozi, L., Ashman, N., Renesto, P., Stanke, N., Lindemann, D., Schoehn, G., and Weissenhorn, W. (2016) Cryo-electron microscopy Structure of the native Prototype Foamy Virus Glycoprotein and virus Architecture. PLoS Pathogens 12(7):e1005721 • C. Caillat, P. Macheboeuf, Y. Wu, A.A. McCarthy, E. Boeri-Erba, G. Effantin, H.G. Gottlinger, W. Weissenhorn and P. Renesto (2015) Asymmetric ring structure of Vps4 required for ESCRT-III disassembly. Nat Commun 6:8781. • M.G. Bego, É. A. Côt, N. Aschman, J. Mercier, W. Weissenhorn and E. A. Cohen (2015) Human Immunodeficiency Virus Accessory Protein Vpu Inhibits the Antiviral Response of Plasmacytoid Dendritic Cells for Innate Immune Evasion. PLoS Pathogens, 11(7):e1005024. • V. Buzon, G. Natrajan, D. Schibli, F. Campelo, M. M. Kozlov, and W. Weissenhorn (2010) Crystal structure of HIV-1 gp41 including both fusion peptide and membrane proximal external regions. PLoS Pathogens, 6(5) e1000880 • A. Hinz, N.Miguet, G. Natrajan, Y. Usami, H. Yamanaka, P. Renesto, B. Hartlieb, A. A. McCarthy, J.-P. Simorre, H. Gottlinger and W. Weissenhorn (2010) Structural basis of HIV-1 tethering to membranes by the Bst2/tetherin ectodomain. Cell Host Microbe, 7, 314-323. • Lata S, Schoehn G, Jain A, Pires R, Piehler J, Gottlinger HG, Weissenhorn W. (2008) Helical structures of ESCRT-III are disassembled by VPS4. Science 321, 1354-1357. • T. Muziol, E. Pineda-Molina, G. Schoehn, R. Ravelli, A. Zamborlini, Y. Usami, H. Göttlinger, and W. Weissenhorn (2006) Structural basis for budding by the ESCRT-III factor CHMP3. Dev. Cell, 10, 821-830. • A. Albertini, A. Wernimont, T. Muziol, R. B. G. Ravelli, C. R. Clapier, G. Schoehn, W. Weissenhorn* and R.W. Ruigrok (2006) Crystal structure of the rabies virus nucleoprotein reveals complete RNA sequestering. Science, 313(5785):360-3. doi : 10.1126/science.1125280. The complete publication list is available here. The complete list of PhD thesis can be found here |