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Institut de Biologie StructuraleGrenoble / France

Integrated Imaging of Stress Response Group (I2SR)

Group leader : Dominique BOURGEOIS

Presentation

The I2SR group investigates stress response mechanisms in prokaryotes and eukaryotes using integrated structural cell biology approaches and in particular, advanced fluorescence imaging techniques. The group is composed of :
The PIXEL Team led by Dominique BOURGEOIS
The DNA Damage and Repair (DDR) Team led by Joanna TIMMINS
The M4D imaging platform led by Jean-Philippe KLEMAN

The group was created through a bottom-up approach, bringing together a methodology-driven team (PIXEL) and a biology-driven team (DDR) to create a real synergy with tight interactions of mutual benefits.
We are interested in cellular responses to stresses such as UV and ionizing radiation and one of our favorite model organisms is Deinococcus radiodurans, a fascinating bacterium with exceptional radiation-resistance capability. We study its remarkable DNA repair machinery and investigate the changes in cell and nucleoid morphology occurring under normal and stress conditions. We also investigate stress response mechanisms in eukaryotic cells, notably in the contexts of cancer and apoptosis. This biological research is tightly intertwined with methodological developments in the fields of fluorescent protein research and advanced fluorescence microscopy, notably single-molecule localization microscopy (SMLM). These developments are of direct benefit to the M4D platform and its users.



Staff

Permanent staff
ADAM Virgile, CNRS CRCN (PIXEL)
BOURGEOIS Dominique, CNRS DR1 (PIXEL)
BYRDIN Martin, CEA E4 (PIXEL)
DE BONIS Salvatore, CEA Tech (DDR)
FRACHET Philippe, UGA MCF HCex (PIXEL)
HANS Fabienne, UGA MCF HC (DDR)
KLEMAN Jean-Philippe, CEA E6 (DDR)
LACROIX Françoise, CNRS IEHC (DDR)
TACNET Pascale, CNRS IR1 (PIXEL)
TIMMINS Joanna, CNRS DR2 (DDR)
VAUCLARE Pierre, CNRS CRCN (DDR)

Ph.D. students and Post-doctoral scientists
BANNEVILLE Anne-Sophie, PhD student (DDR)
DUFOUR Samy, PhD student (PIXEL)
GLUSHONKOV Oleksandr, Post-doc (PIXEL)
MANTOVANELLI Angela, PhD student (PIXEL)
SECK Anna, PhD student (DDR)
WULFFELE Jip, PhD student (PIXEL)

Specialized techniques

Molecular biology & Mutagenesis
Biochemistry (activity assays ; interaction studies ; FRET)
Protein expression & purification
Fluorescent protein engineering
Macromolecular crystallography
Kinetic crystallography
Conventional fluorescence microscopy (spinning disk confocal, video microscopy, FRAP)
Single-molecule localization microscopy (PALM, dSTORM, PAINT), single-particle tracking
Microspectrophotometry

Keywords

Integrated structural cell biology ; Stress response ; Deinococcus radiodurans ; Cancer ; Apoptosis ; Protein dynamics ; Super-resolution fluorescence microscopy ; X-ray crystallography.

Available Services

M4D platform (Jean-Philippe KLEMAN)

Key publications

M. Byrdin, C. Duan, D. Bourgeois, K. Brettel. “A Long-Lived Triplet State Is the Entrance Gateway to Oxidative Photochemistry in Green Fluorescent Proteins”. J. Am. Chem. Soc., (2018), 140, 2897-2905. Doi : 10.1021/jacs.7b12755

E. de Zitter, D. Thédié, V. Mönkemöller, S. Hugelier, J. Beaudouin, V. Adam, M. Byrdin, L. Van Meervelt, P. Dedecker*& D. Bourgeois* .“Mechanistic investigation of mEos4b suggests a strategy to reduce track interruptions in sptPALM”. Nature Meth., (2019) 16, 707-710.

K. Floc’h, F. Lacroix, P. Servant, P. Servant, Y.S. Wong, J.P. Kleman, D. Bourgeois & J. Timmins. “Cell morphology and nucleoid dynamics in dividing
Deinococcus radiodurans”. Nature Commun., (2019), 10, 3815

E. De Zitter, J. Ridard, D. Thédié, V. Adam, B. Lévy, M. Byrdin, G. Gotthard, L. Van Meervelt, P. Dedecker, I. Demachy, and D. Bourgeois*.“Mechanistic Investigations of Green mEos4b Reveal a Dynamic Long-Lived Dark State”. J. Am. Chem. Soc., (2020), 142, 10978–10988. Doi : 10.1021/jacs.0c01880

Publications since 2014 can be found here link to comme soon.