Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / Pebay-Peyroula Eva

Membrane Transport Group

The group aims to understand signalling and transport across biological membrane. To do so, we study membrane proteins at the molecular level with electrophysiology and structural techniques. The membrane transport group comprises four teams :

  • The Gordeliy lab combines an interest in rhodopsins with the development of in meso cristallization
  • The Moreau lab develops the Ion Channel-Coupled Receptor technology
  • The Nury lab focuses on pentameric ligand-gated ion channels
  • The Vivaudou lab studies the molecular mechanisms of ion channels, with a focus on inward-rectifying potassium channels

Publications of the group can be found here.

Key words

  • Ion channels
  • G-Protein Coupled Receptors
  • Potassium channel, Kir channel, K-ATP channel
  • Microorganism rhodopsins
  • Nucleotide transporte
  • Biosensors
  • Serotonin 5-HT3 receptor
  • In meso crystallization

Specialized techniques

  • Overexpression (eukaryotic systems mostly) and purification of membrane proteins
  • Biophysical, biochemical and functional characterizations
  • Membrane protein crystallization with vapor diffusion and lipidic phase (HTMPC platform)
  • X-ray crystallography
  • Electron microscopy
  • Construction of mutants, chimeras, fusion proteins
  • Heterologous expression in Xenopus oocytes (manual and robotic setups)
  • Patch-clamp on cells and proteoliposomes
  • Two-microelectrode voltage clamp (manual and robotic setups)