Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / Pebay-Peyroula Eva

Membrane Transport Group

Group leader : Hugues Nury

The group aims to understand membrane protein activity mechanisms at molecular and atomic level. The group is interested in high resolution structure determination, functional characterization and new approaches for membrane protein crystallization.

The membrane transport group is composed of 2 teams :

Key words

Production, characterization and crystallization of membrane proteins, Nucleotide transporter, AAC, NTT, Cys-loop transporter family, Serotonin receptor 5-HT3, Microorganism rhodopsins, Connexines

Specialized techniques

  • Overexpression (prokaryotes and eukaryotes systems) and purification of membrane proteins
  • Biophysical, biochemical and functional characterizations (thermal stability, FTIR, transport)
  • Membrane protein crystallization with vapor diffusion and lipidic phase (HTMPC platform)
  • X-ray crystallography
  • Electron microscopy

Major publications

- Conformational transitions of the serotonin 5-HT3 receptor, L Polovinkin, G Hassaine, J Perot, E Neumann, A Jensen, S Lefebvre, PJ Corringer, J Neyton, C Chipot, F Dehez, G Schoehn, H Nury, Nature (2018), 563 (7730)

- Mechanism of transmembrane signaling by sensor histidine kinases, I Gushchin, I Melkinov, V Polovinkin, A Ishchenko, A Yuzhakova, P Buslaev, G Bourenkov, S Grudinin, E Round, T Balandin, V Borshchevskiy, D Willbold, G Leonard, G Büldt, A Popov, V Gordeliy,Science (2017), 356 (6342)

- Structural insights into ion conduction by channelrhodopsin 2, O Volkov, K Kovalev, V Polovinkin, V Borshchevskiy, C Bamann, R Astashkin, E Marin, A Popov, T Balandin, D Willbold, G Büldt, E Bamberg, V Gordeliy, Science (2017), 358 (6366)

- Quantification of Detergents Complexed with Membrane Proteins, V Chaptal, F Delolme, A Kilburg, S Magnard, C Montigny, M Picard, C Prier, L Monticelli, O Bornert, M Agez, S Ravaud, C Orelle, R Wagner, A Jawhari, I Broutin, E Pebay-Peyroula, J-M Jault, H. R Kaback, M Le Maire, P Falson, Scientific Reports (2017), 7

- X ray structure of the mouse serotonin 5HT3 receptor, G Hassaine, C Deluz, L Grasso,R Wyss,MB Tol,R Hovius,A Graff, H Stahlberg, T Tomizaki, A Desmyter, C Moreau C, XD Li, F Poitevin, H Vogel, H Nury, Nature (2014), 512 (7514)

- Dangerous liaisons between detergents and membrane proteins. The case of mitochondrial uncoupling protein 2, M Zoonens, J Comer, S Masscheleyn, E Pebay-Peyroula, C Chipot, B Miroux, F Dehez, Journal of the american chemical society (2013), 135 (40)

- Structure of mitochondrial ADP/ATP carrier in complex with carboxyatractyloside, E.Pebay-Peyroula, C. Dahout-Gonzalez, R. Kahn, V. Trézéguet, G.J.-M. Lauquin and G. Brandolin, Nature (2003), 426

(You can find all the publications of the group here).