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Institut de Biologie StructuraleGrenoble / France

Contacts relatifs à cet article / PELLEQUER Jean-Luc

Improve the visibility of AFM images to detect molecular features

To make surface features of TMV visible in the AFM image, the intensity processing involved reduction of stripe noises, histogram equalization, and calculations of mixed partial derivative of pixel intensity, i.e. Dxy2I(x,y) = d2I(x,y)/dxdy, where I(x,y) is the intensity at pixel (x,y).
Using this dedicated image processing, we found that at low resolution (i.e., 1.95 nm per pixel), the extracted surface features of TMV can be interpreted as a partial or full helical repeat (three complete turns with 7.0 nm in length), while individual protein subunits ( 2.5 nm) were perceivable only at high resolution.

Chen S-wW, et al. (2013) Nanoscale structural features determined by AFM for single virus particles. Nanoscale 22 : 10877-10886.