Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / NEUMANN Emmanuelle
Contact person(s) related to this article / Benoit Gallet

Microscope Tecnai 12 (FEI)

Tecnai12 120 kV

The Tecnai12 Microscope operating at 120 kV is dedicated to negative-staining and quality control.
Tomography at Room Temperature is available.

Specificities :
- Tension : 80 - 120 kV
- Electron source : LaB6
- Orius 600 & 1000 GATAN cameras
- Holders : single tilt, FISCHIONE 2020 tomography holder, rotation holder

Microscope Tecnai F20 (FEI)

Tecnai F20 200 kV

The Tecnai F20 transmission electron microscope operating at 200 kV (FEG) is dedicated to cryo-electron microscopy.

Specificities :
- Tension : 80 - 200 kV
- Field Emission Gun (FEG)
- Windows 7
- 4kx4k Ceta FEI camera
- Hybrid pixel detector Cheetah (Amsterdam Scientific Instruments)
- Holders : single tilt, FISCHIONE 2020 tomography holder, 626 GATAN cryo-holder, 914 cryo-tomo holder.

Microscope GLACIOS (Thermo Scientific)


Specificities :
- Tension : 200 kV
- Field Emission Gun (FEG)
- Falcon II Thermo Scientific electron counting direct detection camera
- CETA CMOS camer
- Software : Tomography TEM, EPU (automatic data collection)

December 2018, the IBS received a GLACIOS transmission electron microscope for high-resolution cryo-electron microscopy. The microscope was funded by generous grants from CEA, CNRS, ESRF and FRISBI.
This microscope is a cryo-transmission EM with a 200 kV field emission gun (FEG), which allows imaging of proteins in their native state.
The microscope is equipped with an "AutoLoader" system allowing to load grids. The Autoloader system allows to load up to 12 grids inside the column of the microscope in an automatic way.

To collect high resolution data, the microscope is fitted with one of the highest performance detector available for cryo-electron microscopy : the Falcon II electron counting direct detection camera (Thermo Scientific).

The GLACIOS is also equipped to do single tilt electron tomography with :
- Falcon II camera (direct detection camera)
- Tomography TEM software

The GLACIOS microscope is open to outside (national and international) users after approval of their scientific projects. Trained cryo-electron microscopists have priority but other projects can be accommodated within the framework of collaboration. In any case if you want to use or have an access to the Glacios Microscope or to another equipment of the electron microscopy platform, please send an email to : ibs-plateforme-em.contact@ibs.fr.

Microscope associated instruments

Carbon evaporator

Instrument : Safematic CCU-010
Application : Deposits an homogeneous carbon film onto the surface of a mica sheet or on formvar grids.

Glow discharger

Instrument : Emitech K100X
Application : Makes the surface of the grid hydrophilic/hydrophobic by charging negatively/positively the carbon-coated grid.

Freezing automated system

Instrument : FEI Vitrobot MARK IV
Application : Vitrification of sample by plunge-freezing (preparation of cryo-EM grids).

Instruments for cellular sample preparation

Automated high-pressure freezer

Instrument : Leica EM-HPM100
Application : Cryo-fixation of biological sample under high pressure (2000 Bars).

Automated freeze substitution machine

Instrument : Leica EM-AFS2 + EM-FSP
Application : Freeze substitution process or Progressive Lowering of Temperature (PLT) for ambient or low-temperature embedding of sample, manually or automated with the FSP robot.

(Cryo-)ultra-microtome

Instrument : Leica EM-UC7 + EM-FC7
Application : Ultra-thin sectionning of embedded biological samples, at room temperature or in cryo-conditions.

Automated immuno-labeling machine

Instrument : Leica EM-IGL
Application : Automated staining or immuno-labelling steps on samples on grids (single particles or sections).