Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / FARIAS ESTROZI Leandro

FPM - Fast Projection Matching

FPM brings speed and accuracy to the view determination step in the single-particles 3D reconstruction protocol.

FPM is a FFT-accelerated projection matching method. The electron microscopy images are represented by their Fourier-Bessel transforms and the 3D model by its expansion in spherical harmonics, or more specifically in terms of symmetry-adapted functions. The rotational and translational properties of these representations are used to quickly access all the possible 2D projections of the 3D model, which allow an exhaustive inspection of the whole five-dimensional domain of parameters associated to each particle.

References :

1 - Estrozi LF & Navaza J, "Fast projection matching for cryo-electron microscopy image reconstruction", Journal of Structural Biology, 162, p.324–334, 2008.
2 - Estrozi LF & Navaza J, "Ab initio high-resolution single-particle 3D reconstructions : The symmetry adapted functions way", Journal of Structural Biology, 172, p.253–260, 2010.