Accueil > Research > Research groups > Structure and Activity of Glycosaminoglycans Group (H.Lortat-Jacob) > SAGAG / Lortat-Jacob Team
Presentation of Lortat-Jacob TeamScientific activityThe team is developing two lines of research aimed at (1) analyzing the structure-function relationships of protein-glycosaminoglycan interactions, using mainly cytokines or pathogens as experimental models, and (2) characterizing the mechanisms determining the functional oligosaccharide sequences of HS by studying the biosynthetic machinery of these molecules. Team membersCurrent members Alumni Main publications (2009-2019)• Debarnot C., Monneau Y.R., Roig-Zamboni V., Delauzun V., Le Narvor C., Richard E., Hénault J., Goulet A., Fadel F., Vivès R.R., Priem B., Bonnaffé D., Lortat-Jacob H., Bourne Y. Structural insights into substrate binding and catalytic mechanism of human heparan sulfate D-glucuronyl C5 epimerase. • Richter R.P. and Lortat-Jacob H. Ménage à trois with glycosaminoglycans - a serious rendezvous, not a gag ! • Monneau Y.R., Luo L., Sankaranarayanan N.V., Nagarajan B., Vivès R.R., Baleux F., Desai U.R., Arenzana-Seidedos F. and Lortat-Jacob H. Solution structure of CXCL13 and heparan sulphate binding show that GAG binding site and biological activity rely on distinct domains. • Connell B.J., Sadir R., Baleux F., Laguri C., Kleman J-P., Luo L., Arenzana-Seisdedos F. and Lortat-Jacob H. Heparan Sulfate differently regulates CXCL12α and CXCL12γ mediated chemotaxis through differential presentation to CXCR4. • Arien K.K., Baleux F., Desjardins D., Porrot F., Coic Y.-M., Michiels J., Bouchemal K., Bonnaffé D., Bruel T., Schwartz O., Le Grand R., Vanham G., Dereuddre-Bosquet N. and Lortat-Jacob H. CD4-mimetic sulfopeptide conjugates display sub-nanomolar anti-HIV-1 activity and protect macaques against a SHIV162P3 vaginal challenge. • Monneau Y., Arenzana-Seisdedos F. and Lortat-Jacob H. The sweet spot : how GAGs help chemokines guide migrating cells. • Préchoux A., Halimi C., Simorre J.P., Lortat-Jacob H. and Laguri C. C5-epimerase and 2-O-sulfotransferase associate in vitro to generate contiguous epimerized and 2-O-sulfated heparan sulfate domains. • Dalonneau F., Liu X.Q., Sadir R., Almodovar J., Mertani H.C., Bruckert F., Albiges-Rizo C., Weidenhaupt M., Lortat-Jacob H. and Picart C. The effect of delivering the chemokine SDF-1α in a matrix-bound manner on myogenesis. • Saesen E., Sarrazin S., Laguri C., Sadir R., Maurin D., Thomas A., Imberty A. and Lortat-Jacob H. Insights into the mechanism by which Interferon-gamma basic amino acid clusters mediate protein binding to heparan sulfate. J. Am. Chem. Soc. 135, 9384−9390 (2013) • Sarris M., Masson J.B., Maurin D., Van der Aa L.M., Boudinot P., Lortat-Jacob H. and Herbomel P. Chemokines guide neutrophil interstitial migration by imposing a haptotactic biased random walk. • Hou Y., Genua M., Tada Batista D., Calemczuk R., Buhot A., Fornarelli P., Koubachi J., Bonnaffé D., Saesen E., Laguri C., Lortat-Jacob H. and Livache T. Continuous Evolution Profiles for Electronic Tongue Based Analysis. • Lortat-Jacob H., Burhan I., Scarpellini A., Thomas A., Imberty A., Vivès RR., Johnson T., Gutierrez A., and Verderio E.A.M. Transglutaminase-2 interaction with heparin : Identification of a heparin binding site that regulates cell adhesion to fibronectin-transglutaminase-2 matrix. J. Biol. Chem. 287, 18005-18017 (2012) • Connell B.J., Baleux F., Coic YM., Clayette P., Bonnaffé D and Lortat-Jacob H. A synthetic heparan sulfate-mimetic peptide conjugated to a mini CD4 displays very high anti-HIV-1 activity independently of coreceptor usage. • Laguri C., Sapay N., Simorre J.P., Brutscher B. Imberty A., Gans P. and Lortat-Jacob H. 13C-labeled heparan sulfate analogue as a tool to study protein/heparan sulfate interaction by NMR spectroscopy. Application to the CXCL12α chemokine. • Boldajipour B., Doitsidou M., Tarbashevich K., Laguri C., Yu S.R., Ries J., Dumstrei K., Thelen S., Dörries J., Messerschmidt E-M., Thelen M., Schwille P., Brand M., Lortat-Jacob H., Raz E. Control of CXCL12 function by ligand subfunctionalization. • Lortat-Jacob H. The molecular basis and functional implications of chemokine interactions with heparan sulphate. • Baleux F., Loureiro-Morais L., Hersant Y., Clayette P., Arenzana-Seisdedos F., Bonnaffé B. Lortat-Jacob H. A synthetic CD4-HS glycoconjugate inhibits both CCR5 and CXCR4 HIV-1 attachment and entry. Scientific collaborations• Dr. D. Bonnaffé, ICMMO, Orsay |
