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Institut de Biologie StructuraleGrenoble / France

Contact person(s) related to this article / PETOSA Carlo

Presentation

MOLECULAR MECHANISMS OF INFECTION AND DISEASE

Team leader : Carlo Petosa

Our team is broadly interested in understanding the molecular mechanisms that underlie infectious disease. Current work focuses on :

- Inhibiting epigenetic targets in pathogenic fungi
- Nucleocytoplasmic transport of HIV proteins
- Nucleosome and chromatin structure

Our approach is to study the structure and function of key proteins involved in these processes and to use the insights gained to develop new strategies for the treatment and prevention of disease.

The team also hosts the Mass spectrometry (MS) platform, which offers various services and performs research on macromolecular complexes .


Team members :

- Laura Belot (Postdoc)
- Elisabetta Boeri Erba (CEA Research Engineer)
- Ramachandran Boopathi (Postdoc)
- Hasan Dawi (Master’s student)
- Raphaël Dupeyron (Ph.D. student)
- Isabel García Sáez (CNRS Researcher)
- Jordan Hayat (Master’s student)
- Rose Laure Indorato (CNRS Technician)
- Marjolaine Noirclerc-Savoye (CEA Research Engineer)
- Carlo Petosa (CNRS Research Director)
- Luca Signor (CEA Research Engineer)
- Dimitrios Skoufias (CEA Researcher)
- Kaiyao Wei (Ph.D. student)

Techniques :

- Protein expression and purification
- Biochemical and biophysical characterization of macromolecular complexes
- Molecular and cell biology
- X-ray crystallography
- Electron microscopy
- Mass spectrometry

Recent publications :

2020
Boopathi R, Dimitrov S, Hamiche A, Petosa C, Bednar J.
Cryo-electron microscopy of the chromatin fiber.
Curr Opin Struct Biol. 64:97-103, 2020

Puglisi R, Boeri Erba E, Pastore A.
A Guide to Native Mass Spectrometry to determine complex interactomes of molecular machines.
FEBS Journal 287 : 2428-2439, 2020

Rasmussen KK, Palencia A, Varming AK, El-Wali H, Boeri Erba E, Blackledge M, Hammer K, Torsten Herrmann, Kilstrup M, Lo Leggio L, and Jensen MR.
Revealing the mechanism of repressor inactivation during switching of a temperate bacteriophage.
Proc Nat Acad Sci, in press, 2020

Boeri Erba E, Signor L, Petosa C.
Exploring the structure and dynamics of macromolecular complexes by native mass spectrometry.
J Proteomics, 222:103799, 2020

Boopathi R, Danev R, Khoshouei M, Kale S, Nahata S, Ramos L, Angelov D, Dimitrov S, Hamiche A, Petosa C, Bednar J.
Phase-plate cryo-EM structure of the Widom 601 CENP-A nucleosome core particle reveals differential flexibility of the DNA ends.
Nucleic Acids Res, 48:5735-5748, 2020

2019
Indorato RL, Talapatra SK, Lin F, Haider S, Mackay SP, Kozielski F, Skoufias DA.
Is the fate of clinical candidate Arry-520 already sealed ? Predicting resistance in Eg5-inhibitor complexes.
Mol Cancer Ther. 18:2394-2406, 2019

Ben Fadhel N, Signor L, Petosa C, Noirclerc-Savoye M.
Phosphomimetic mutations modulate the ability of HIV-1 Rev to bind human Importin-beta in vitro.
Matters. doi : 10.19185/matters.201903000032, 2019

Moulin M, Mossou E, Signor L, Kieffer-Jaquinod S, Kwaambwa HM, Nermark F, Gutfreund P, Mitchell EP, Haertlein M, Forsyth VT, Rennie AR.
Towards a molecular understanding of the water purification properties of Moringa seed proteins.
J Colloid Interface Sci. 554:296-304, 2019

Torra J, Lafaye C, Signor L, Aumonier S, Flors C, Shu X, Nonell S, Gotthard G, Royant A.
Tailing miniSOG : structural bases of the complex photophysics of a flavin-binding singlet oxygen photosensitizing protein.
Sci Rep. 9(1):2428, 2019

2018
Garcia-Saez I, Menoni H, Boopathi R, Shukla MS, Soueidan L, Noirclerc-Savoye M, Le Roy A, Skoufias DA, Bednar J, Hamiche A, Angelov D, Petosa C, Dimitrov S.
Structure of an H1-bound 6-nucleosome array reveals an untwisted two-start chromatin fiber conformation.
Mol Cell, 72:902-915, 2018

Boeri Erba E, Signor L, Oliva MF, Hans F, Petosa C.
Characterising intact macromolecular complexes using native mass spectrometry.
Methods Mol Biol, 1764:133-151, 2018

2017
Mietton F, Ferri E, Champleboux M, Zala N, Maubon D, Zhou Y, Harbut M, Spittler D, Garnaud C, Chauvel M, d’Enfert C, Kashemirov BA, Hull M, Cornet M, McKenna CE, Govin J, Petosa C.
Selective BET bromodomain inhibition as an antifungal therapeutic strategy.
Nature Comm 8:15482, 2017

Bednar J, Garcia-Saez I, Tonchev O, Cutter A, Papai G, Reymer A, Syed SH, Lone IN, Boopathi R, Crucifix C, Menoni H, Papin C, Skoufias D, Kurumizaka H, Lavery R, Hamiche A, Hayes JJ, Schultz P, Angelov D, Petosa C, Dimitrov S.
Structure and dynamics of a 197 base-pair nucleosome in complex with linker histone H1.
Mol Cell 66:384-397, 2017

2016
Coscia F, Estrozi LF, Hans F, Malet H, Noirclerc-Savoye M, Schoehn G, Petosa C.
Fusion to a homo-oligomeric scaffold allows cryo-EM analysis of a small protein.
Sci. Reports. 6:30909. doi : 10.1038/srep30909, 2016.

Goudarzi A, Zhang D, Huang H, Barral S, Kwon O, Qi S, Tang Z, Buchou T, Vitte AL, He T, Cheng Z, Montellier E, Gaucher J, Curtet S, Debernardi A, Charbonnier G, Puthier D, Petosa P, Panne D, Rousseaux S, Roeder RG, Zhao Y, Khochbin S.
Dynamic competing histone H4 K5K8 acetylation and butyrylation are hallmarks of highly active gene promoters.
Mol Cell 62:169-180, 2016

Meyer PM, Socias S, Key J, Ransey E, Tjon E, Maia F, Buschiazzo A, Lei M, Botka C, Withrow J, Neau D, Rajashankar K, Anderson K, Baxter RH, Blacklow SC, Boggon TJ, Bonvin AMJJ, Borek D, Brett TJ, Caflisch A, Chang C-I, Chazin WJ, Corbett KD, Cosgrove MS, Crosson S, Dhe-Paganon S, Di Cera E, Drennan C, Eck M, Eichman BF, Fan QR, Ferré-D’Amaré AR, Fraser JS, Fromme JC, Garcia KC, Gaudet R, Gong P, Harrison SC, Heldwein EE, Jia Z, Keenan RJ, Kruse AC, Kvansakul M, McLellan JS, Modis Y, Otwinowski Z, Pai EF, Pereira P, Petosa C, Raman CS, Rapoport TA, Roll-Mecak A, Rosen MK, Rudenko G, Schlessinger J, Schwartz TU, Shamoo Y, Sondermann H, Tao YJ, Tolia N, Tsodikov OV, Westover KD, Wu H, Foster I, Maia FRNC, Gonen T, Kirchausen T, Diederichs K, Crosas M, Sliz P.
Data publication with the structural biology data grid supports live analysis.
Nature Comm 7:10882, 2016

Ferri E, Petosa C, McKenna CE.
Bromodomains : structure, function and pharmacology of inhibition.
Biochem Pharmacol 2016, 106:1-18.

2015
Morozumi Y, Boussouar F, Tan M, Chaikuad A, Jamshidikia M, Colak G, He H, Nie L, Petosa C, de Dieuleveult, Curtet S, Vitte AL, Schweifer N, Gianni D, Gut M, Guardiola P, Rousseaux S, Gérard M, Knapp S, Zhao Y, Khochbin S.
Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells.
J Mol Cell Biol Oct 12. pii : mjv060, 2015.

Boeri Erba E, Petosa C.
The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular assemblies.
Protein Sci. 24:1176-92, 2015