IBS - Institut de Biologie Structurale - Grenoble / France

Team members

2022-11-04T14:37:48Z

Members

Yvain Nicolet (Head, CEA Research Director)
Nikolas Alansson(PhD Student)
Yogen Borkar (PhD student))
Mickaël Cherrier (UGA Assistant Professor)
Eugenio de la Mora (CEA Researcher)
Tuan Anh Dinh (PhD student)
Juan Fontecilla-Camps (Head from 1992 to 2015, CEA Research Director until 2021, now CEA Scientific Advisor)
Lydie Martin (CEA Engineer)
Rahila Rahimova (Post-doc)
Maria Cristina Stefanoiu (PhD student))
Xavier Vernède (CEA Engineer)

Alumni

Jean-François Couture (Visiting scientist)
Rayan Osman (Post-doctoral fellow)
Jérémie Ruel (2021: PhD Student, Master 2 student)
Juneina Omeiri (PhD Student)
Maria Cristina Stefanoiu (Master 1 student)
Ali Hnaino (Master 2 student)
Damien Lafond-Fenonjoie (Master 1 student)
Tu Quynh Nguyen (PhD Student)
Eda Nur Kavlak (L3 student)
Amandine Barbaroux (L3 student)
Nkene Owone Olivia (Master 1 student)
Roman Rohac (Post-doctoral fellow)
Leon Jenner (Post-doctoral fellow)
Julie Michelon (L2 student)
Noëlie Palermo (L3 student)
Maelys Baudin (BTS trainee)
Fanny Lanoé (Master 1 student)
Ti-My Phuong Do (Master 2 student)
Rania Khouja (L3 student)
Mathias Eymery (Master 2 student)
Raleb Taher (PhD Student)
Maxime Bella (PhD Student)
Adriano Lopes Rodrigues (Master 2 student)
Anh Nguyen (Master 2 student)
Mathieu Viennois (L3 student)
Chuchu Guo (L3 student)
Camille Larosa (Master 1 student)
Amélie Vessière (BTS trainee)
Roman Rohac (PhD student)
Adrien Pagnier (PhD student)
Pablo Rado (Master 2 student)
Jade Reboulet (BTS trainee)
Karen Rihani (Master 2 student)
Mélanie Rodà (L1 student)
Imen Khtatfi (Master 2 Student)
Mohamed Ben Amor (Master 1 Student)
Widade Ziani (PhD Student)
Elodie Marinoni (PhD Student)
Hugo Lebrette (PhD Student)

Former members since 2014

Patricia Amara (CEA Researcher from 2008 to 2026)
Anne Volbeda (CEA Resarcher from 1992 to 2023, CEA Scientific Advisor until 2025)
Eve de Rosny (UGA Assistant Professor from 2004 to 2025)
Alexandre Smith-Naura (Technician)
Anthony Usclat (Technician)
Lucia Paya Tormo (Collaborator)
Fabien Chenavier (Technician)
David Fernandez-Remolar (Visiting Scientist)
Claire Saragaglia (Technician)
Ana Sosa Fajardo (Assistant Engineer)
Clara Rinaldi (Technician)
Claudine Darnault (CEA Technician)
Océane Gigarel (Technician)
Lucia Paya Tormo (Visiting PhD Student)
Viviam Moura da Silva (Visiting PhD Student)
Oriane Renoux (Technician)
Mónica Navarro Rodriguez (Visiting PhD Student)
Laura Zeppieri (Technician)
Myriam Savard (Technician)

Theses

2022-11-04T11:34:27Z

All the theses from the group

The list of PhD theses undertaken in the Metalloproteins' group can be found year by year below (manual list).

2025

Jérémie Ruel

Biosynthesis of darobactin, a novel antibiotic against gram negative pathogens. Thèse de doctorat de l'Université Grenoble Alpes, soutenue le 3 Février 2025.

2024

Juneina Omeiri

Assembly machinery of the [FeFe]-hydrogenase active site. Thèse de doctorat de l'Université Grenoble Alpes, soutenue le 10 Décembre 2024.

Tu Quynh Nguyen

Assembly machinery of the nitrogenase active site. Thèse de doctorat de l'Université Grenoble Alpes, soutenue le 12 Janvier 2024.

2018

Raleb Taher

Etude de l'activation du système Zra : régulation de l'activation de ZraS par Zrap la protéine accessoire du système. Thèse de doctorat de l'Université Grenoble Alpes, soutenue le 16 novembre 2018.

2016

Roman Rohac

Etude Structurale et fonctionnelle de la protéine à radical SAM HydE. Thèse de doctorat de l'Université Grenoble Alpes, soutenue le 18 mai 2016.

2015

Adrien Pagnier

Maturation de sites métalliques de protéines par les machineries d'assemblage des centres fer-soufre ISC et Hyd. Thèse de doctorat de l'Université Grenoble Alpes, soutenue le 2 novembre 2015.

2014

Widade Ziani

Interactions des protéines du complexe transduction du signal transmembranaire
CnrYXH chez Cupriavidus metallidurans CH34. Thèse de doctorat de l'Université Grenoble Alpes, soutenue le 18 décembre 2014.

2013

Hugo Lebrette
Etude structurale de l'import de nickel par les protéines extracytoplasmiques de systèmes ABC chez les bactéries. Thèse de doctorat de l'Université Grenoble Alpes, soutenue le 04 octobre 2013.

2011

Elodie Marinoni
Maturation de sites métalliques de protéines par les protéines à radical S-Adénosyl-L-méthionine et la machinerie de fabrication des centres fer-soufre. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, soutenue le 09 décembre 2011.

Juliette Trepreau
Perception du stress métallique (nickel/cobalt) par le système de signalisation transmembranaire Cnr chez Cupriavidus metallidurans CH34. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, soutenue le 18 novembre 2011.

2006

Mickaël Cherrier
Etude cristallographique des protéines NikA et NikR impliquées dans le transport du nickel chez Escherichia coli. Quand la structure de NikA met en évidence l'existence possible d'un nouveau métallophore. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, soutenue le 05 mai 2006. [thèse en ligne]

2005

Jérôme Dupuy
Etude cristallographique de la protéine IRP1, un régulateur de l'homéostasie du fer. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, soutenue le 31 mai 2005. [thèse en ligne]

2004

Lynn Gregory
Etude structurale des protéases des complexes initiant la défense immunitaire par la voie du complément.Thèse de doctorat de l'Université Joseph Fourier, Grenoble.

2001

Yvain Nicolet
Etude cristallographique de l'hydrogénase à fer de la bactérie sulfato-réductrice Desulfovibrio desulfuricans ATCC 7757. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, soutenue le 29 juin 2001.

2000

Arnaud Gruez
Etude cristallographique de la sulfite reductase : mise en evidence d'un module flavodoxine desordonne. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, 2000.

Renaud Moralès
Association et dissociation de deux protéines photosynthétique : étude cristallographique du complexe Ferredoxine-NADP+ reductase/Ferredoxine chez Anabaena PCC 7119. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, soutenue le 13 décembre 2000.

1999

Eric Chabrière
Étude structurale de la pyruvate ferrédoxine oxydoreductase de Desulfovibrio africanus par cristallographie des rayons X : vers une meilleure connaissance du mécanisme réactionnel du cofacteur thiamine pyrophosphate. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, soutenue le 7 juillet 1999.

1998

Elsa Garcin
Études cryocristallographiques du site actif des hydrogénases à nickel de Desulfovibrio gigas et Desulfomicrobium baculatum. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, soutenue le 19 juin 1998.

Yaël Montet
Élucidation des mécanismes d'action des hydrogénases à Ni-Fe : étude de l'hydrogénase de Desulfovibrio fructosovorans et de ses mutant par cristallographie des rayons X. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, soutenue le 30 octobre 1998.

1997

Catherine Mazza
Hydroxysteroide deshydrogenase humaine de type i : analyse des relations structure-fonction par mutagenese dirigee et cristallographie des rayons x. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, 1997.

Joachim Hirschler
Influence des impuretés sur la cristallisation d'une protéine modèle dans différents milieux. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, 1997.

1996

Denis Verger
Etude cristallographiqu.e preliminaire de la region globulaire de c1q et d'un fragment de c3 du complement humain. Structures cristallines de complexes entre la subtilisine de bacillus lentus et des inhibiteurs de type acide boronique. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, 1996.

1994

David Pignol
Structures cristallines de la sous-unité III du complexe procarboxypeptidase A-S6 de boeuf et du zymogène E de porc. Thèse de doctorat de l'Université Joseph Fourier, Grenoble I, soutenue le 17 juin 1994.

Access the full-content of the theses from the group

If you want more details, note that the HAL-IBS website publishes the full content of thesis that are not under embargo or under a particular contractual obligation. The result is listed below, the display may take 1 to 2 seconds.
Please note that the theses of the last 12 months are not yet available on this site and theses prior to 2010 are not exhaustively listed.

Publications

2022-11-03T17:34:20Z

Publications since 2014

Our list of publications with peerReviewing, books and book sections since 2014 can be found below (data issued from HAL-IBS).
The display may take 1 to 2 seconds. To check the entire list, use the inner scroll bar.

Publications until 2013

Our list of publications from 2000 to 2013 is displayed below.

Publications 2013

Bachmeier A, Wang VCC, Woolerton TW, Bell S, Fontecilla-Camps JC, Can M, Ragsdale SW, Chaudhary YS and Armstrong FA
How light-harvesting semiconductors can alter the bias of reversible electrocatalysts in favor of H-2 production and CO2 reduction.
Journal of the American Chemical Society (2013) 135(40): 15026-15032

Mickaël V. Cherrier, Sylvain Engilberge, Patricia Amara, Alice Chevalley, Michèle Salmain, and Juan C. Fontecilla-Camps
Structural Basis for Enantioselectivity in the Transfer Hydrogenation of a Ketone Catalyzed by an Artificial Metalloenzyme
Eur.J.Inorg.Chem. 2013, 3596-3600

Chenevier P, Mugherli L, Darbe S, Darchy L, DiManno S, Tran PD, Valentino F, Iannello M, Volbeda A, Cavazza C and Artero V
Hydrogenase enzymes: Application in biofuel cells and inspiration for the design of noble-metal free catalysts for H2 oxidation.
Comptes Rendus Chimie (2013) 16(5): 491-505

Esmieu C, Cherrier MV, Amara P, Girgenti E, Marchi-Delapierre C, Oddon F, Iannello M, Jorge-Robin A, Cavazza C and Ménage S
Oxygenase built from scratch: substrate binding site identified using a docking approach.
Angewandte Chemie International Edition (2013) 52(14): 3922-3925

Evans RM, Parkin A, Roessler MM, Murphy BJ, Adamson H, Lukey MJ, Sargent F, Volbeda A, Fontecilla-Camps JC and Armstrong FA
Principles of sustained enzymatic hydrogen oxidation in the presence of oxygen - The crucial influence of high potential Fe-S clusters in the electron relay of [NiFe]-hydrogenases.
Journal of the American Chemical Society (2013) 135(7): 2694-2707

Gaboriaud C, Gupta RK, Martin L, Lacroix M, Serre L, Teillet F, Arlaud GJ, Rossi V and Thielens NM
The serine protease domain of MASP-3 : enzymatic properties and crystal structure in complex with ecotin.
PLoS One (2013) 8(7):e67962

Heidari Khajepour MY, Lebrette H, Vernede X, Rogues P and Ferrer J-L
A geometrical approach for semi-automated crystal centering and in situ X-ray diffraction data collection.
Journal of Applied Crystallography (2013) 46: 740-745

Heidari Khajepour MY, Vernede X, Cobessi D, Lebrette H, Rogues P, Terrien M, Berzin C and Ferrer J-L
REACH: Robotic Equipment for Automated Crystal Harvesting using a six-axis robot arm and a micro-gripper.
Acta Crystallographica Section D-Biological Crystallography (2013) 69: 381-387

Lebrette H, Iannello M, Fontecilla-Camps JC and Cavazza C
The binding mode of Ni-(L-His)2 in NikA revealed by X-ray crystallography.
Journal of Inorganic Biochemistry (2013) 121: 16-18

Mouesca JM, Amara P, Fontecilla-Camps JC
Electronic States of the O2-tolerance [NiFe] hydrogenase proximal cluster.
Proceedings of the National Academy of Sciences of the United States of America (2013) 110: E2538

Mouesca J-M, Fontecilla-Camps JC and Amara P
The structural plasticity of the proximal [4Fe3S] cluster is responsible for the O2 tolerance of membrane-bound [NiFe] hydrogenases.
Angewandte Chemie International Edition (2013) 52: 2002-2006

Nachon F, Carletti E, Ronco C, Trovaslet M, Nicolet Y, Jean L, Renard PY
Crystal structures of human cholinesterases in complex with huprine W and tacrine: elements of specificity for anti-Alzheimer's drugs targeting acetyl- and butyryl-cholinesterase.
Biochemical Journal (2013) 453: 392-399

Nicolet Y, Rohac R, Martin L and Fontecilla-Camps JC
X-ray snapshots of possible intermediates in the time course of synthesis and degradation of protein-bound Fe4S4 clusters.
Proceedings of the National Academy of Sciences (2013) 110(18): 7188-7192

Volbeda A, Amara P, Iannello M, DeLacey AL, Cavazza C, Fontecilla-Camps JC
Structural foundations for the O2 resistance of Desulfomicrobium baculatum [NiFeSe]-hydrogenase.
Chemical Communications (2013) 49: 7061-7063

Volbeda A, Darnault C, Parkin A, Sargent F, Armstrong FA and Fontecilla-Camps JC
Crystal structure of the O2-tolerant membrane-bound hydrogenase 1 from Escherichia coli in complex with its cognate cytochrome b.
Structure (2013) 21(1): 184-190

Yamanaka Y, Zeppieri L, Nicolet Y, Marinoni EN, de Oliveira JS, Odaka M, Dean DR and Fontecilla-Camps JC
Crystal structure and functional studies of an unusual l-cysteine desulfurase from Archaeoglobus fulgidus.
Dalton Transactions (2013) 42: 3092-3099

Publications 2012

Chan A, Clemancey M, Mouesca JM, Amara P, Hamelin O, Latour JM and Ollagnier de Choudens S
Studies of inhibitor binding to the [4Fe-4S] cluster of quinolinate synthase.
Angewandte Chemie International Edition (2012) 51(31): 7711-7714

Cherrier MV, Girgenti E, Amara P, Iannello M, Marchi-Delapierre C, Fontecilla-Camps JC, Menage S and Cavazza C
The structure of the periplasmic nickel-binding protein NikA provides insights for artificial metalloenzyme design.
Journal of Biological Inorganic Chemistry (2012) 17(5): 817-829

de Rosny E and Carpentier P
GFP-Like phototransformation mechanisms in the cytotoxic fluorescent protein killerRed unraveled by structural and spectroscopic investigations.
Journal of the American Chemical Society (2012) 134(43): 18015-18021

Maitre M, Weidmann S, Rieu A, Fenel D, Schoehn G, Ebel C, Coves J and Guzzo J
The oligomer plasticity of the small heat-shock protein Lo18 from Oenococcus oeni influences its role in both membrane stabilization and protein protection.
Biochemical Journal (2012) 444(1): 97-104

Marinoni EN, de Oliveira JS, Nicolet Y, Raulfs EC, Amara P, Dean DR and Fontecilla-Camps JC
(IscS-IscU)2 complex structures provide insights into Fe2 S2 biogenesis and transfer.
Angewandte Chemie International Edition (2012) 51(22): 5439-5442

Nicolet Y and Fontecilla-Camps JC
Structure-function relationships in [FeFe]-hydrogenase active site maturation.
Journal of Biological Chemistry (2012) 287(17): 13532-13540

Volbeda A, Amara P, Darnault C, Mouesca JM, Parkin A, Roessler MM, Armstrong FA and Fontecilla-Camps JC
X-ray crystallographic and computational studies of the O2-tolerant [NiFe]-hydrogenase 1 from Escherichia coli.
Proceedings of the National Academy of Sciences (2012) 109(14): 5305-5310

Publications 2011

Amara P, Mouesca JM, Volbeda A and Fontecilla-Camps JC
Carbon monoxide dehydrogenase reaction mechanism: A likely case of abnormal CO₂ insertion to a Ni−H⁻
bond
Inorganic Chemistry (2011) 50: 1868-1878

Cavazza C, Martin L, Laffly E, Lebrette H, Cherrier MV, Zeppieri L, Richaud P, Carriere M and Fontecilla-Camps JC
Histidine 416 of the periplasmic binding protein NikA is essential for nickel uptake in Escherichia coli.
FEBS Letters (2011) 585 (4): 711-715

Nachon F, Carletti E, Wandhammer M, Nicolet Y, Schopfer LM, Masson P and Lockridge O
X-ray crystallographic snapshots of reaction intermediates in the G117H mutant of human butyrylcholinesterase, a nerve agent target engineered into a catalytic bioscavenger.
Biochemical Journal (2011) 434(1): 73-82

Smirnov I, Carletti E, Kurkova I, Nachon F, Nicolet Y, Mitkevich VA, Debat H, Avalle B, Belogurov AA, Kuznetsov N, Reshetnyak A, Masson P, Tonevitsky AG, Ponomarenko N, Makarov AA, Friboulet A, Tramontano A and Gabibov A
Reactibodies generated by kinetic selection couple chemical reactivity with favorable protein dynamics.
Proceedings of the National Academy of Sciences of the United States of America (2011) 108(38): 15954-15959

Trepreau J, Girard E, Maillard AP, Rosny Ed, Petit-Haertlein I, Kahn R and Covès J
Structural basis for metal sensing by CnrX.
Journal of Molecular Biology (2011) 408 (4): 766-779

Tron C, Cherrier MV, Amara P, Martin L, Fauth F, Fraga E, Correard M, Fontecave M, Nicolet Y and Fontecilla-Camps JC
Further characterization of the [FeFe]-Hydrogenase Maturase HydG.
European Journal of Inorganic Chemistry (2011): 1221-1227

Wait AF, Brandmayr C, Stripp ST, Cavazza C, Fontecilla-Camps JC, Happe T and Armstrong FA
Formaldehyde-A rapid and reversible inhibitor of hydrogen production by [FeFe]-hydrogenases.
Journal of the American Chemical Society (2011) 133 (5): 1282-1285

Wandhammer M, Carletti E, Van der Schans M, Gillon E, Nicolet Y, Masson P, Goeldner M, Noort D and Nachon F
Structural study of the complex stereoselectivity of human butyrylcholinesterase for the neurotoxic V-agents.
Journal of Biological Chemistry (2011) 286(19): 16783-16789

Publications 2010

Cavazza C, Bochot C, Rousselot-Pailley P, Carpentier P, Cherrier MV, Martin L, Marchi-Delapierre C, Fontecilla-Camps JC and Menage S
Crystallographic snapshots of the reaction of aromatic C-H with O₂ catalysed by a protein-bound iron complex.
Nature Chemistry (2010) 2(12): 1069-1076

Fontecilla-Camps JC
A short essay on the probability of the existence of life and intelligence elsewhere in the universe.
Human Evolution (2010) 25: 173-188

Laffly E, Garzoni F, Fontecilla-Camps JC and Cavazza C
Maturation and processing of the recombinant [FeFe] hydrogenase from Desulfovibrio vulgaris Hildenborough (DvH) in Escherichia coli.
International Journal of Hydrogen Energy (2010) 35(19): 10761-10769

Liebgott PP, Leroux F, Burlat B, Dementin S, Baffert C, Lautier T, Fourmond V, Ceccaldi P, Cavazza C, Meynial-Salles I, Soucaille P, Fontecilla-Camps JC, Guigliarelli B, Bertrand P, Rousset M and Leger C
Relating diffusion along the substrate tunnel and oxygen sensitivity in hydrogenase.
Nature Chemical Biology (2010) 6(1): 63-70

Nicolet Y, Fontecilla-Camps JC and Fontecave M
Maturation of [FeFe]-hydrogenases: Structures and mechanisms.
International Journal of Hydrogen Energy (2010) 35(19): 10750-10760
3.452

Nicolet Y, Martin L, Tron C and Fontecilla-Camps JC
A glycyl free radical as the precursor in the synthesis of carbon monoxide and cyanide by the [FeFe]-hydrogenase maturase HydG.
Febs Letters (2010) 584(19): 4197-4202
3.541

Schauer K, Muller C, Carriere M, Labigne A, Cavazza C and De Reuse H
The Helicobacter pylori GroES co-chaperonine HspA functions as a specialized nickel-chaperone and sequestration protein through its unique C-terminal extension.
Journal of Bacteriology (2010) 192(5): 1231-7

Volbeda A, Nicolet Y and Fontecilla-Camps JC
The RNA world and the origin of life: an ancient protein fold links metal-based gas reactions with the RNA world.
Journal of Cosmology (2010) 10: 3243-3257

Publications 2009

Dementin S, Leroux F, Cournac L, de Lacey AL, Volbeda A, Leger C, Burlat B, Martinez N, Champ S, Martin L, Sanganas O, Haumann M, Fernandez VM, Guigliarelli B, Fontecilla-Camps JC and Rousset M
Introduction of methionines in the gas channel makes [NiFe] hydrogenase aero-tolerant.
Journal of the American Chemical Society (2009) 131(29): 10156-10164

Fontecilla Camps JC
Structure and Function of [NiFe]-hydrogenases.
Metal Ions in Life Sciences (2009) 6: 151-178

Fontecilla-Camps JC, Amara P, Cavazza C, Nicolet Y and Volbeda A
Structure-function relationships of anaerobic gas-processing metalloenzymes.
Nature (2009) 460(7257): 814-822

Goldet G, Brandmayr C, Stripp S, Happe T, Cavazza C, Fontecilla Camps JC and Armstrong FA
Electrochemical kinetic investigations of the reactions of [FeFe]-hydrogenases with carbon monoxide and oxygen: comparing the importance of gas tunnels and active-site electronic/redox effects.
Journal of the American Chemical Society (2009) 131(41): 14979–14989

Nicolet Y, Amara P, Mouesca JM and Fontecilla-Camps JC
Unexpected electron transfer mechanism upon AdoMet cleavage in radical SAM proteins.
Proceeding of the National Academy of Sciences USA (2009) 106(35): 14867-14871

Pilet E, Nicolet Y, Mathevon C, Douki T, Fontecilla-Camps JC and Fontecave M
The role of the maturase HydG in [FeFe]-hydrogenase active site synthesis and assembly.
FEBS Letters (2009) 583(3): 506-511

Reisner EF, Fontecilla-Camps JC and Armstrong FA
Catalytic electrochemistry of a [NiFeSe]-hydrogenase on TiO₂ and demostration of its suitability for visible-light driven H2 production.
Chemical Communications (2009) 5: 550-552

Rousselot-Pailley P, Bochot C, Marchi-Delapierre C, Jorge-Robin A, Martin L, Fontecilla-Camps JC, Cavazza C and Menage S
The protein environment drives selectivity for sulfide oxidation by an artificial metalloenzyme.
Chembiochem (2009) 10(3): 545-552

Volbeda A, Darnault C, Tan X, Lindahl PA and Fontecilla-Camps JC
Novel domain arrangement in the crystal structure of a truncated acetyl-CoA synthase from Moorella thermoacetica.
Biochemistry (2009) 48(33): 7916-7926

Publications 2008

Armstrong FA and Fontecilla-Camps JC
Biochemistry. A natural choice for activating hydrogen.
Science (2008) 321(5888): 498-499

Carletti E, Li H, Li B, Ekstrom F, Nicolet Y, Loiodice M, Gillon E, Froment MT, Lockridge O, Schopfer LM, Masson P and Nachon F
Aging of cholinesterases phosphylated by tabun proceeds through o-dealkylation.
Journal of the American Chemical Society (2008) 130(47): 16011-16020

Cavazza C, Martin L, Mondy S, Gaillard J, Ratet P and Fontecilla-Camps JC
The possible role of an [FeFe]-hydrogenase-like protein in the plant responses to changing atmospheric oxygen levels.
Journal of Inorganic Biochemistry (2008) 102(5-6): 1359-1365

Cherrier MV, Cavazza C, Bochot C, Lemaire D and Fontecilla-Camps JC
Structural characterization of a putative endogenous metal chelator in the periplasmic nickel transporter NikA.
Biochemistry (2008) 47(38): 9937-9943

Fdez Galvan I, Volbeda A, Fontecilla-Camps JC and Field MJ
A QM/MM study of proton transport pathways in a [NiFe] hydrogenase.
Proteins (2008) 73(1): 195-203

Leroux F, Dementin S, Burlat B, Cournac L, Volbeda A, Champ S, Martin L, Guigliarelli B, Bertrand P, Fontecilla-Camps J, Rousset M and Leger C
Experimental approaches to kinetics of gas diffusion in hydrogenase.
Proceedings of the National Academy of Sciences U S A (2008) 105(32): 11188-11193

Nicolet Y, Rubach JK, Posewitz MC, Amara P, Mathevon C, Atta M, Fontecave M and Fontecilla-Camps JC
X-ray structure of the [fefe]-hydrogenase maturase hydE from Thermotoga maritima.
Journal of Biological Chemistry (2008) 283: 18861-18872

Parkin A, Goldet G, Cavazza C, Fontecilla-Camps JC and Armstrong FA
The difference a Se makes? Oxygen-Tolerant hydrogen production by the [NiFeSe]-hydrogenase from Desulfomicrobium baculatum
Journal of the American Chemical Society (2008) 130(40): 13410-13416

Publications 2007

Amara P, Fdez Galvan I, Fontecilla-Camps JC and Field MJ
The enamine intermediate may not be universal to thiamine catalysis.
Angewandte Chemie International Edition England (2007) 46(47): 9019-9022

Chandor A, Douki T, Gasparutto D, Gambarelli S, Sanakis Y, Nicolet Y, Ollagnier-de-Choudens S, Atta M and Fontecave M
Characterization of the DNA repair spore photoproduct lyase enzyme from Clostridium acetobutylicum: A radical-SAM enzyme.
Comptes Rendus Chimie (2007) 10(8): 756-765

Fioravanti E, Vellieux FM, Amara P, Madern D and Weik M
Specific radiation damage to acidic residues and its relation to their chemical and structural environment.
Journal of Synchrotron Radiation (2007) 14(Pt 1): 84-91

Fontecilla-Camps JC, Volbeda A, Cavazza C and Nicolet Y
Structure/function relationships of [NiFe]- and [FeFe]-hydrogenases.
Chemical Review (2007) 107(10): 4273-4303

Katona G, Carpentier P, Niviere V, Amara P, Adam V, Ohana J, Tsanov N and Bourgeois D
Raman-assisted crystallography reveals end-on peroxide intermediates in a nonheme iron enzyme.
Science (2007) 316(5823): 449-453

Morales R, Berna A, Carpentier P, Contreras-Martel C, Renault F, Nicodeme M, Chesne-Seck ML, Bernier F, Dupuy J, Schaeffer C, Diemer H, Van-Dorsselaer A, Fontecilla-Camps JC, Masson P, Rochu D and Chabriere E
[Discovery and crystallographic structure of human apolipoprotein].
Annales Pharmaceutiques Françaises (2007) 65(2): 98-107

Razavet M, Artero V, Cavazza C, Oudart Y, Lebrun C, Fontecilla-Camps JC and Fontecave M
Tricarbonylmanganese(I)-lysosyme complex: A structurally characterized organometallic protein.
Chemical Communications (2007) 2007(27): 2805-2807

Publications 2006

Amara P and Serre L
Functional flexibility of Bacillus stearothermophilus formamidopyrimidine DNA-glycosylase
DNA repair (2006) 5: 947-958.

Cavazza C, Contreras-Martel C, Pieulle L, Chabriere E, Hatchikian EC and Fontecilla-Camps JC
Flexibility of thiamine diphosphate revealed by kinetic crystallographic studies of the reaction of pyruvate-ferredoxin oxidoreductase with pyruvate.
Structure (2006) 14(2): 217-224

Contreras-Martel C, Carpentier P, Morales R, Renault F, Chesne-Seck ML, Rochu D, Masson P, Fontecilla-Camps JC and Chabriere E
Crystallization and preliminary X-ray diffraction analysis of human phosphate-binding protein.
Acta Crystallographica Section F: Structural Biology and Crystallization Communications (2006) 62(Pt 1): 67-69

Dupuy J, Volbeda A, Carpentier P, Darnault C, Moulis JM and Fontecilla-Camps JC
Crystal structure of human iron regulatory protein 1 as cytosolic aconitase.
Structure (2006) 14(1): 129-139

Morales R, Berna A, Carpentier P, Contreras-Martel C, Renault F, Nicodeme M, Chesne-Seck ML, Bernier F, Dupuy J, Schaeffer C, Diemer H, Van-Dorsselaer A, Fontecilla-Camps JC, Masson P, Rochu D and Chabriere E
Serendipitous discovery and x-ray structure of a human phosphate binding apolipoprotein.
Structure (2006) 14(3): 601-609

Parkin A, Cavazza C, Fontecilla-Camps JC and Armstrong FA
Electrochemical investigations of the interconversions between catalytic and inhibited states of the [FeFe]-hydrogenase from Desulfovibrio desulfuricans.
Journal of the American Chemical Society (2006) 128(51): 16808-16815

Pecqueur L, D'Autréaux B, Dupuy J, Nicolet Y, Jacquamet L, Brutscher B, Michaud-Soret I and Bersch B
Structural changes of Escherichia coli ferric uptake regulator during metal-dependent dimerization and activation explored by NMR and x-ray crystallography.
Journal of Biological Chemistry (2006) 281(30): 21286-21295

Tan X, Volbeda A, Fontecilla-Camps J and Lindahl P
Function of the tunnel in acetylcoenzyme A synthase/carbon monoxide dehydrogenase.
Journal of Biological Inorganic Chemistry (2006) 11(3): 371-378

Volbeda A and Fontecilla-Camps JC
Catalytic nickel-iron clusters : from minerals to enzymes.
Topics in Organometallic Chemistry (2006) 17: 57-82

Walden WE, Selezneva AI, Dupuy J, Volbeda A, Fontecilla-Camps JC, Theil EC and Volz K
Structure of dual function iron regulatory protein 1 complexed with ferritin IRE-RNA.
Science (2006) 314(5807): 1903-1908

Publications 2005

Amara P, Volbeda A, Fontecilla-Camps JC and Field MJ
A quantum chemical study of the reaction mechanism of acetyl-coenzyme a synthase
Journal of the American Chemical Society (2005) 127(8): 2776-2784

Cherrier M, Martin L, Cavazza C, Jacquamet L, Lemaire D, Gaillard J and Fontecilla-Camps JC
Crystallographic and spectroscopic evidence for high affinity binding of FeEDTA(H2O)^- to periplasmic nickel transporter NikA
Journal of the American Chemical Society (2005) 127(28): 10075-10082

Dupuy J, Darnault C, Brazzolotto X, Kuhn LC, Moulis JM, Volbeda A and Fontecilla-Camps JC
Crystallization and preliminary X-ray diffraction data for the aconitase form of human iron-regulatory protein 1.
Acta Crystallographica Section F Structural Biology and Crystallization Communications (2005) 61(Pt 5): 482-485

Tormos JR, Wiley KL, Seravalli J, Nachon F, Masson P, Nicolet Y and Quinn DM
The reactant state for substrate-activated turnover of acetylthiocholine by butyrylcholinesterase is a tetrahedral intermediate.
Journal of the American Chemical Society (2005) 127(42): 14538-14539

Vincent KA, Parkin A, Lenz O, Albracht SP, Fontecilla-Camps JC, Cammack R, Friedrich B and Armstrong FA
Electrochemical definitions of O2 sensitivity and oxidative inactivation in hydrogenases.
J Am Chem Soc (2005) 127(51): 18179-18189

Volbeda A and Fontecilla-Camps JC
Structure-function relationships of nickel-iron sites in hydrogenase and a comparison with the active sites of other nickel-iron enzymes.
Coordination Chemistry Reviews (2005) 249(15-16): 1609-1619

Volbeda A and Fontecilla-Camps JC
Structural bases for the catalytic mechanism of Ni-containing carbon monoxide dehydrogenases.
Dalton Transactions (2005) (21): 3443-3450

Volbeda A, Martin L, Cavazza C, Matho M, Faber BW, Roseboom W, Albracht SPJ, Garcin E, Rousset M and Fontecilla-Camps JC
Structural differences between the ready and unready oxidized states of [NiFe] hydrogenases
Journal of biological Inorganic Chemistry (2005) 10(3): 239-249

Publications 2004

Amara P, Serre L, Castaing B and Thomas A
Insights into the DNA repair process by the formamidopyrimidine-DNA glycosylase investigated by molecular dynamics
Protein Science (2004) 13: 2009-2021

Volbeda A and Fontecilla-Camps J C
Crystallographic evidence for a CO/CO(2) tunnel gating mechanism in the bifunctional carbon monoxide dehydrogenase/acetyl coenzyme A synthase from Moorella thermoacetica
Journal Of Biological Inorganic Chemistry (2004) 9(5): 525-532

Publications 2003

Amara P and Field M J
Evaluation of an ab initio quantum mechanical/molecular mechanical hybrid-potential link-atom method
Theoretical Chemistry Accounts (2003) 109(1): 43-52

Darnault C, Volbeda A, Kim E J, Legrand P, Vernede X, Lindahl P A and Fontecilla-Camps J C
Ni-Zn-[Fe(4)-S(4)] and Ni-Ni-[Fe(4)-S(4)] clusters in closed and open alpha subunits of acetyl-CoA synthase/carbon monoxide dehydrogenase.
Nature Structural Biology (2003) 10(4): 271-279

Loudwig S, Nicolet Y, Masson P, Fontecilla Camps J C, Bon S, Nachon F and Goeldner M
Photoreversible inhibition of cholinesterases: Catalytic serine-labeled caged butyrylcholinesterase
Chembiochem (2003) 4(8): 762-767

Nicolet Y, Lockridge O, Masson P, Fontecilla-Camps J C and Nachon F
Crystal structure of human butyrylcholinesterase and of its complexes with substrate and products
The Journal Of Biological Chemistry (2003) 278(42): 41141-41147

Volbeda A and Fontecilla Camps J C
The active site and catalytic mechanism of [NiFe] hydrogenases.
Dalton Transactions (2003) 21: 4030-4038

Publications 2002

David L, Amara P, Field M J and Major F
Parametrization of a force field for metals complexed to Biomacromolecules : applications to Fe(II), Cu(II) and Pb(II)
Journal of Computer Aided Molecular Design (2002) 16: 635-651

Frey M
Hydrogenases: Hydrogen-activating enzymes
Chembiochem (2002) 3(2-3): 153-160

Hurley J K, Morales R, Martinez Julvez M, Brodie T B, Medina M, Gomez Moreno C and Tollin G
Structure-function relationships in Anabaena ferredoxin/ferredoxin : NADP(+) reductase electron transfer: insights from site-directed mutagenesis, transient absorption spectroscopy and X-ray crystallography
Biochimica Et Biophysica Acta Bioenergetics (2002) 1554(1-2): 5-21

Morales R, Frey M and Mouesca J M
An approach based on quantum chemistry calculations and structural analysis of a [2Fe-2S*] ferredoxin that reveal a redox-linked switch in the electron-transfer process to the Fd-NADP(+) reductase.
Journal of the American Chemical Society (2002) 124(23): 6714-6722

Nachon F, Nicolet Y, Viguie N, Masson P, Fontecilla Camps JC and Lockridge O
Engineering of a monomeric and low-glycosylated form of human butyrylcholinesterase - Expression, purification, characterization and crystallization
European Journal of Biochemistry (2002) 269(2): 630-637

Nicolet Y, Cavazza C and Fontecilla-Camps J C
Fe-only hydrogenases: structure, function and evolution
Journal of Inorganic Biochemistry (2002) 91(1): 1-8

Stadler C, De Lacey A L, Montet Y, Volbeda A, Fontecilla-Camps J C, Conesa J C and Fernandez V M
Density functional calculations for modeling the active site of nickel- iron hydrogenases. 2. predictions for the unready and ready states and the corresponding activation processes.
Inorganic Chemistry (2002) 41(17): 4424-4434

Publications 2001

Amara, P., Andreoletti, P., Jouve, H. M. and Field, M. J.
Ligand diffusion in the catalase from Proteus mirabilis: A molecular dynamics study.
Protein Science (2001) 10(10): 1927-1935

Chabriere E., Vernede X., Guigliarelli B., Charon M. H., Hatchikian E. C. and Fontecilla-Camps J. C.
Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase.
Science (2001) 294(5551): 2559-63

Contreras Martel C, Martinez Oyanedel J, Bunster M, Legrand P, Piras C, Vernede X and Fontecilla Camps JC
Crystallization and 2.2 angstrom E resolution structure of R-phycoerythrin from Gracilaria chilensis: a case of perfect hemihedral twinning
Acta Crystallographica Section D Biological Crystallography (2001) 57 Part 1: 52-60

Nicolet Y, de Lacey AL, Vernede X, Fernandez VM, Hatchikian EC and Fontecilla Camps JC
Crystallographic and FTIR spectroscopic evidence of changes in Fe coordination upon reduction of the active site of the Fe-only hydrogenase from Desulfovibrio desulfuricans
Journal of the American Chemical Society (2001) 123(8): 1596-1601

Publications 2000

Amara P, Field MJ, Alhambra C and Gao JL
The generalized hybrid orbital method for combined quantum mechanical/molecular mechanical calculations: formulation and tests of the analytical derivatives
Theoretical Chemistry Accounts (2000) 104(5): 336-343

Gruez, A, Pignol, D, Zeghouf, M, Coves, J, Fontecave, M, Ferrer, JL and Fontecilla-Camps, JC
Four crystal structures of the 60 kDa flavoprotein monomer of the sulfite reductase indicate a disordered flavodoxin-like module [In Process Citation].
Journal of Molecular Biology (2000) 299(1): 199-212

Hemmingsen L, Amara P, Ansoborlo E and Field MJ
Importance of charge transfer and polarization effects for the modeling of uranyl-cation complexes
Journal of Physical Chemistry A (2000) 104(17): 4095-4101

Morales, R., Charon, M. H., Kachalova, G., Serre, L., Medina, M., Gomez Moreno, C. and Frey, M.
A redox-dependent interaction between two electron-transfer partners involved in photosynthesis.
Embo Reports (2000) 1(3): 271-276

Morales, R., Kachalova, G., Vellieux, F., Charon, M. H. and Frey, M.
Crystallographic studies of the interaction between the ferredoxin- NADP+ reductase and ferredoxin from the Cyanobacterium Anabaena: looking for the elusive ferredoxin molecule.
Acta Crystallographica D Biological Crystallography (2000) 56(Pt 11): 1408-1412

Morales, R., M. H. Charon, G. Hudry-Clergeon, Y. Petillot, S. Norager, M. Medina and M. Frey
Refined X-ray structures of the oxidized, at 1.3 A, and reduced, at 1.17 A, [2Fe-2S] ferredoxin from the Cyanobacterium Anabaena PCC7119 Show Redox-Linked Conformational Changes.
Biochemistry (2000) 39(9): 2428

Morelli, X., M. Czjzek, C. E. Hatchikian, O. Bornet, J. C. Fontecilla-Camps, P. N. Palma, J. J. Moura and F. Guerlesquin
Structural model of the Fe-hydrogenase-cytochrome c553 complex combining TROSY experiments and soft docking calculations.
Journal of Biological Chemistry (2000) 275(30): 23204-10

Nicolet, I., B. J. Lemon, J Fontecilla-Camps and J.W. Peters
A novel FeS cluster in Fe-only hydrogenases [In Process Citation].
Trends in Biochemical Science (2000) 25(3): 138-43

Presentation

Nicolet Yvain — 2011-01-07T15:27:04Z

Group Leader: Yvain Nicolet , recipient of the Dr and Mme Henri Labbé Prize

Metals lie at the heart of chemical processes that are at the origin of life, explaining their crucial role in the function of numerous important proteins. Their specific properties enable catalytic processes that proteins alone cannot achieve. Conversely, these properties may be tuned by the protein environment offering unique reactivity beyond synthetic compounds. The Metalloproteins Unit explores these critical roles in biology.

The Metalloproteins Unit focuses on understanding the structure-function relationships of oxygen-sensitive metalloenzymes. Using X-ray crystallography and/or single particle cryogenic electron microscopy, combined with computational methods, we primarily investigate iron-sulfur cluster-containing enzymes that play crucial roles in bioenergetic primary metabolism, the biosynthesis of (metallo)-cofactors or the production of antibiotics. The majority of the investigated proteins are very sensitive to oxygen, necessitating their study under anaerobic conditions inside glove boxes. Consequently, we continue to develop methodological approaches to enable access to cutting-edge structural biology techniques in an oxygen-free environment, with the specific aim of exploring the dynamic aspects of the processes we study.

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Specialized techniques and available services

Manipulations under controlled atmospheres using glove boxes for anaerobic cell growth, protein purification, manual crystallization, automated crystallization, crystal manipulation and automated crystal visualization, cryo-EM sample preparation.

IBS - Institut de Biologie Structurale - Grenoble / France

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