Group presentation

<multi>[fr]logo de l'équipe[en]logo of the team</multi>
logo FRM 2017 avec image de microscopy de bacterie et boites de petri differents couleur

KEY WORDS

Pseudomonas aeruginosa,Francisella tularensis, Klebsiella pneumoniae, antibiotic resistence, toxins, host-pathogen interaction, regulation, signaling, nosocomial infection, anti-virulence

RESEARCH TOPICS

  • P. aeruginosa virulence factors
    * Type III secretion system – structure, function and inhibition
    * Mechanisms of action of ExoU – intracellular traficking
    * Two partner secretion (TPS) proteins - ExlA and PdtA
  • P. aeruginosa regulatory networks
    * two component systems involved in virulence and biofilms
  • Bacterial resistence to immune system – persistence mechanisms (Pseudomonas and Klebsiella)
  • P. aeruginosa enveloppe
    * Resistence to antimicrobial peptides (AMPs)
    * Study of the elongasome / divisome
  • Virulence of Francisella tularensis
    * Mechanisms of escape from the innate immune system
    * Virulence facteur : Type VI secretion system

SPECIFIC TECHNIQUES

  • Bacterial genetics (engineering of KO mutants)
  • Cellular bacteriology
  • Genome-wide screens (CRISPR-Cas and Transposon)
  • NGS (Tn-Seq, WGS, DAP-Seq, CHIP-Seq, RNA-Seq...)
  • Microscopy (Fluorescence microscopy, IF, 3D video, confocal spinning-disk microscopy, automated microscopy and image analysis)
  • Host-pathogen interaction (infection models eg: Galleria mellonella, macrophages, epithelial cells and human blood)
  • Intra-bacterial competition assay (E. coli, Pseudomonas sp, Acinetobacter, Bacillus sp…)
  • FACS (cytokines measurements, detection and quantification of surface proteins, T3SS toxins injection test using beta-lactamase reporter system, sorting of cells and bacteria)
  • Protein expression and purification (in E. coli and P. aeruginosa),
  • Protein-protein and protein-lipid interactions, lipid raft purification

COLLABORATIONS

  • Harvard Medical School, Boston, US (Steve Lory)
  • Department of Medical Microbiology, Ultrecht, NE (Susan H.M. Rooijakkers)
  • Institut Pasteur, Paris (Carmen Buchrieser)
  • Equipe I2BA ’"Inflammation, infections bactériennes et auto-inflammation", CIRI, Lyon (Thomas Henry)
  • Département de Pharmacochimie Moléculaire, Grenoble (Yung-Sing Wong)
  • Nano-Optics and Forces Team, Institut Néel, Grenoble (Jochen Fick)
  • CHU Avicenne, Bobigny (B. Lamy)
  • IAB, Grenoble (A. Palencia)
  • LGP2, Grenoble (N. Reverdy-Bruas, A. Denneulin)
  • Cermav, Grenoble (Sami Halila)
  • TrEE Team, TIMC LAB, Grenoble (Audrey Le Gouellec)
  • CEA/IRIG/Symmes, Grenoble (Thierry Livache)
  • CEA-LETI, Grenoble (P. Marcoux)
  • « Gen&Chem » Team, Grenoble (Marie-Odile Fauvarque)
  • EDyP-Service, Grenoble (Yohann Couté)
  • IBS, Grenoble (Andrea Dessen and Pascal Poignard)