![<multi>[fr]logo de l'équipe[en]logo of the team</multi>](IMG/png/logo_pbrc_300.png){kind=logo}
KEY WORDS
Pseudomonas aeruginosa,Francisella tularensis, Klebsiella pneumoniae, antibiotic resistence, toxins, host-pathogen interaction, regulation, signaling, nosocomial infection, anti-virulence
RESEARCH TOPICS
- P. aeruginosa virulence factors
* Type III secretion system – structure, function and inhibition
* Mechanisms of action of ExoU – intracellular trafficking
* Two partner secretion (TPS) proteins - ExlA and PdtA - P. aeruginosa regulatory networks
* two component systems involved in virulence and biofilms - Bacterial resistance to immune system – persistence mechanisms (Pseudomonas and Klebsiella)
- P. aeruginosa envelope
* Resistence to antimicrobial peptides (AMPs)
* Study of the elongasome / divisome - Virulence of Francisella tularensis
* Mechanisms of escape from the innate immune system
* Virulence factors : Type VI secretion system - Monoclonal antibodies (mAbs) targeting bacterial virulence factors
Pseudomonas aeruginosa avec ses principaux facteur de virulences
SPECIFIC TECHNIQUES
- Bacterial genetics (engineering of KO mutants)
- Cellular bacteriology
- Genome-wide screens (CRISPR-Cas and Transposon)
- NGS (Tn-Seq, WGS, DAP-Seq, CHIP-Seq, RNA-Seq...)
- Microscopy (Fluorescence microscopy, IF, 3D video, confocal spinning-disk microscopy, automated microscopy and image analysis)
- Host-pathogen interaction (infection models eg : Galleria mellonella, macrophages, epithelial cells and human blood)
- Intaer-bacterial competition assay (E. coli, Pseudomonas sp, Acinetobacter, Bacillus sp…)
- FACS (cytokines measurements, detection and quantification of surface proteins, T3SS toxins injection test using beta-lactamase reporter system, sorting of cells and bacteria)
- Protein expression and purification (in E. coli and P. aeruginosa),
- Protein-protein and protein-lipid interactions, lipid raft purification
COLLABORATIONS
- Harvard Medical School, Boston, US (Steve Lory)
- Department of Medical Microbiology, Ultrecht, NE (Susan H.M. Rooijakkers)
- Institut Pasteur, Paris (Carmen Buchrieser)
- Equipe I2BA ’"Inflammation, infections bactériennes et auto-inflammation", CIRI, Lyon (Thomas Henry)
- Département de Pharmacochimie Moléculaire, Grenoble (Yung-Sing Wong)
- Nano-Optics and Forces Team, Institut Néel, Grenoble (Jochen Fick)
- CHU Avicenne, Bobigny (B. Lamy)
- IAB, Grenoble (A. Palencia)
- LGP2, Grenoble (N. Reverdy-Bruas, A. Denneulin)
- Cermav, Grenoble (Sami Halila)
- TrEE Team, TIMC LAB, Grenoble (Audrey Le Gouellec)
- CEA/IRIG/Symmes, Grenoble (Thierry Livache)
- CEA-LETI, Grenoble (P. Marcoux)
- « Gen&Chem » Team, Grenoble (Marie-Odile Fauvarque)
- EDyP-Service, Grenoble (Yohann Couté)
- IBS, Grenoble (Andrea Dessen and Pascal Poignard)