Group presentation

<multi>[fr]logo de l'équipe[en]logo of the team</multi>
logo FRM 2017 avec image de microscopy de bacterie et boites de petri differents couleur


Pseudomonas aeruginosa, toxins, host-pathogen interaction, regulation, signaling, cystic fibrosis, nosocomial infection, anti-virulence


  • P. aeruginosa virulence factors
    * Type III secretion system – structure, function and inhibition
    * Mechanisms of action of ExoU – intracellular traficking
    * Two partner secretion (TPS) proteins - ExlA and PdtA
  • P. aeruginosa regulatory networks
    * two component systems involved in virulence and biofilms
  • Bacterial resistence to immune system – persistence mechanisms (Pseudomonas and Klebsiella)
  • P. aeruginosa enveloppe


  • Bacterial genetics (engineering of KO mutants)
  • Cellular bacteriology
  • Genome-wide screens (CRISPR-Cas and Transposon)
  • NGS (Tn-Seq, WGS, DAP-Seq, CHIP-Seq, RNA-Seq...)
  • Microscopy (Fluorescence microscopy, IF, 3D video, confocal spinning-disk microscopy, automated microscopy and image analysis)
  • Host-pathogen interaction (infection models eg : Galleria mellonella, macrophages, epithelial cells and human blood)
  • Intra-bacterial competition assay (E. coli, Pseudomonas sp, Acinetobacter, Bacillus sp…)
  • FACS (cytokines measurements, detection and quantification of surface proteins, T3SS toxins injection test using beta-lactamase reporter system, sorting of cells and bacteria)
  • Protein expression and purification (in E. coli and P. aeruginosa),
  • Protein-protein and protein-lipid interactions, lipid raft purification


  • Harvard Medical School, Boston, US (Steve Lory)
  • Department of Medical Microbiology, Ultrecht, NE (Susan H.M. Rooijakkers)
  • Institut Pasteur, Paris (Carmen Buchrieser)
  • « Gen&Chem » Team, Grenoble (Marie-Odile Fauvarque)
  • EDyP-Service, Grenoble (Yohann Couté)
  • Département de Pharmacochimie Moléculaire, Grenoble (Yung-Sing Wong)
  • IBS, Grenoble (Andrea Dessen and Pascal Poignard)


Postdoctoral research position financed by the ANR (National Research Agency) (from September 2023).