![<multi>[fr]logo de l'équipe[en]logo of the team</multi>](IMG/png/logo_pbrc_300.png)

KEY WORDS
Pseudomonas aeruginosa, toxins, host-pathogen interaction, regulation, signaling, cystic fibrosis, nosocomial infection, anti-virulence
RESEARCH TOPICS
- P. aeruginosa virulence factors
* Type III secretion system – structure, function and inhibition
* Mechanisms of action of ExoU – intracellular traficking
* Two partner secretion (TPS) proteins - ExlA and PdtA - P. aeruginosa regulatory networks
* two component systems involved in virulence and biofilms - Bacterial resistence to immune system – persistence mechanisms (Pseudomonas and Klebsiella)
- P. aeruginosa enveloppe
SPECIFIC TECHNIQUES
- Bacterial genetics (engineering of KO mutants)
- Cellular bacteriology
- Genome-wide screens (CRISPR-Cas and Transposon)
- NGS (Tn-Seq, WGS, DAP-Seq, CHIP-Seq, RNA-Seq...)
- Microscopy (Fluorescence microscopy, IF, 3D video, confocal spinning-disk microscopy, automated microscopy and image analysis)
- Host-pathogen interaction (infection models eg : Galleria mellonella, macrophages, epithelial cells and human blood)
- Intra-bacterial competition assay (E. coli, Pseudomonas sp, Acinetobacter, Bacillus sp…)
- FACS (cytokines measurements, detection and quantification of surface proteins, T3SS toxins injection test using beta-lactamase reporter system, sorting of cells and bacteria)
- Protein expression and purification (in E. coli and P. aeruginosa),
- Protein-protein and protein-lipid interactions, lipid raft purification
COLLABORATIONS
- Harvard Medical School, Boston, US (Steve Lory)
- Department of Medical Microbiology, Ultrecht, NE (Susan H.M. Rooijakkers)
- Institut Pasteur, Paris (Carmen Buchrieser)
- « Gen&Chem » Team, Grenoble (Marie-Odile Fauvarque)
- EDyP-Service, Grenoble (Yohann Couté)
- Département de Pharmacochimie Moléculaire, Grenoble (Yung-Sing Wong)
- IBS, Grenoble (Andrea Dessen and Pascal Poignard)
JOB POSITION
Postdoctoral research position financed by the ANR (National Research Agency) (from September 2023).