Group presentation

KEY WORDS

Pseudomonas aeruginosa, toxins, host-pathogen interaction, regulation, signaling, cystic fibrosis, nosocomial infection, anti-virulence

RESEARCH TOPICS

• P. aeruginosa virulence factors
  * Type III secretion system – structure, function and inhibition
  * Mechanisms of action of ExoU – intracellular traficking
  * Two partner secretion (TPS) proteins - ExlA and PdtA
• P. aeruginosa regulatory networks
  * two component systems involved in virulence and biofilms
• Bacterial resistence to immune system – persistence mechanisms (Pseudomonas and Klebsiella)
• P. aeruginosa enveloppe

SPECIFIC TECHNIQUES

• Bacterial genetics (engineering of KO mutants)
• Cellular bacteriology
• Genome-wide screens (CRISPR-Cas and Transposon)
• NGS (Tn-Seq, WGS, DAP-Seq, CHIP-Seq, RNA-Seq...)
• Microscopy (Fluorescence microscopy, IF, 3D video, confocal spinning-disk microscopy, automated microscopy and image analysis)
• Host-pathogen interaction (infection models eg : Galleria mellonella, macrophages, epithelial cells and human blood)
• Intra-bacterial competition assay (E. coli, Pseudomonas sp, Acinetobacter, Bacillus sp…)
• FACS (cytokines measurements, detection and quantification of surface proteins, T3SS toxins injection test using beta-lactamase reporter system, sorting of cells and bacteria)
• Protein expression and purification (in E. coli and P. aeruginosa),
• Protein-protein and protein-lipid interactions, lipid raft purification

COLLABORATIONS

• Harvard Medical School, Boston, US (Steve Lory)
• Department of Medical Microbiology, Ultrecht, NE (Susan H.M. Rooijakkers)
• Institut Pasteur, Paris (Carmen Buchrieser)
• « Gen&Chem » Team, Grenoble (Marie-Odile Fauvarque)
• EDyP-Service, Grenoble (Yohann Couté)
• Département de Pharmacochimie Moléculaire, Grenoble (Yung-Sing Wong)
• IBS, Grenoble (Andrea Dessen and Pascal Poignard)

JOB POSITION

Postdoctoral research position financed by the ANR (National Research Agency) (from September 2023).