Group leader : Hugues Nury
The group aims to understand signaling and transport across a biological membrane. To do so, we study membrane proteins at the molecular level with electrophysiology and structural techniques. The membrane transport group comprises four teams :
- The Gordeliy lab combines an interest in rhodopsins with the development of in meso crystallization
- The Moreau lab develops the Ion Channel-Coupled Receptor technology
- The Nury lab focuses on pentameric ligand-gated ion channels
- The Vivaudou lab studies the molecular mechanisms of ion channels, with a focus on inward-rectifying potassium channels
Publications of the group can be found here.
Key words
- Ion channels
- G-Protein Coupled Receptors
- Potassium channel, Kir channel, K-ATP channel
- Microorganism rhodopsins
- Nucleotide transporte
- Biosensors
- Serotonin 5-HT3 receptor
- In meso crystallization
Specialized techniques
- Overexpression (eukaryotic systems mostly) and purification of membrane proteins
- Biophysical, biochemical and functional characterizations
- Membrane protein crystallization with vapor diffusion and lipidic phase (HTMPC platform)
- X-ray crystallography
- Electron microscopy
- Construction of mutants, chimeras, and fusion proteins
- Heterologous expression in Xenopus oocytes (manual and robotic setups)
- Patch-clamp on cells and proteoliposomes
- Two-microelectrode voltage clamp (manual and robotic setups)